association of gst genes polymorphisms with asthma in tunisian children协会的gst基因多态性与突尼斯儿童的哮喘.pdfVIP

Hindawi Publishing Corporation Mediators of Inflammation Volume 2007, Article ID 19564, 6 pages doi:10.1155/2007/19564 Clinical Study Association of GST Genes Polymorphisms with Asthma in Tunisian Children 1 1 1, 2 1 ` 1, 2 Chelbi Hanene, Lachheb Jihene, Ammar Jamel, Hamzaoui Kamel, and Hamzaoui Agnes 1 Homeostasis and Cell Dysfunction Unit Research 99/UR/08-40, Faculty of Medicine, University of Tunis El Manar II, Tunis 1007, Tunisia 2 Department of Respiratory and Pediatric Diseases, Pneumology Hospital A. Mami, Ariana 2080, Tunisia Received 8 November 2006; Revised 26 January 2007; Accepted 27 January 2007 Background. A positive association between genetic polymorphism and asthma may not be extrapolated from one ethnic group to another based on intra- and interethnic allelic and genotype frequencies differences. Objective. We assessed whether polymor- phisms of GST genes (GSTM1, GSTT1, and GSTP1) are associated with asthma and atopy among Tunisian children. Methods. 112 unrelated healthy individuals and 105 asthmatic (73 atopic and 32 nonatopic) children were studied. Genotyping the poly- morphisms in the GSTT1 and GSTM1 genes was performed using the multiplex PCR. The GSTP1 ILe105Val polymorphism was determined using PCR-RFLP. Results. GSTM1 null genotype was significantly associated with the increased risk of asthma (P = .002). Asthmatic children had a higher prevalence of the GSTP1Ile105 allele than the control group (43.8% and 33.5%, respectively; P = .002). Also, the presence of the GSTP1 homozygote Val/Val was less common in subjects with asthma than in control group. We have found that GSTT1 null genotype (GSTT1 ∗ 0/∗ 0) was significant