effect of starvation-refeeding status on cholesterol metabolism in rats fed high-cholesterol dietstarvation-refeeding状态对胆固醇代谢的影响的老鼠喂高胆固醇的饮食.pdfVIP

Food and Nutrition Sciences, 2011, 2, 118-123 doi:10.4236/fns.2011.22016 Published Online April 2011 (/journal/fns) Effect of Starvation-Refeeding Status on Cholesterol Metabolism in Rats Fed High-Cholesterol Diet 1 2 Reiko Inai , Tatsuhiro Matsuo 1Department of Health and Nutritional Science, Nagoya Keizai University, Aichi, Japan; 2Faculty of Agriculture, Kagawa University, Kagawa, Japan. Email: matsuo@ag.kagawa-u.ac.jp Received November 9th, 2010; revised January 3rd, 2011; accepted January 24th, 2011. ABSTRACT The present study investigated the effect of starvation-refeeding status on cholesterol metabolism in rats fed a high-cholesterol diet or a cholesterol-free diet. Twenty male and 20 female Donryu rats (age 5 weeks) were fed a cho- lesterol-free diet for 14 days. Then the male and female rats were each divided into two groups: feeding and starva- tion-refeeding groups. The feeding groups were fed the experimental diet for 3 days, and the starvation-refeeding groups fasted for 2 days followed by 3 days of feeding. Half of each of groups was fed a cholesterol-free diet and the other half was fed a high-cholesterol diet. Starvation-refeeding significantly increased the plasma free cholesterol and HDL-cholesterol concentrations in both the high-cholesterol-diet-fed rats and the cholesterol-free-diet-fed rats. In the female rats , plasma total cholesterol and cholesteryl ester c oncentrations were significantly higher in the high-cholesterol groups than in the cholesterol-free groups, whereas TG concentration and total cholesterol/TG ratio were not significantly different among all of the groups. Liver total cholesterol and cholesteryl ester were significantly higher in the high-cholesterol groups than in the cholesterol-free groups in both male and female rats. These results suggest