effect of steric constraint at the γ-backbone position on the conformations and hybridization properties of pnas的空间约束效应γ-backbone在pnas的构象和杂交性质上的立场.pdfVIP

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effect of steric constraint at the γ-backbone position on the conformations and hybridization properties of pnas的空间约束效应γ-backbone在pnas的构象和杂交性质上的立场.pdf

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effect of steric constraint at the γ-backbone position on the conformations and hybridization properties of pnas的空间约束效应γ-backbone在pnas的构象和杂交性质上的立场

SAGE-Hindawi Access to Research Journal of Nucleic Acids Volume 2011, Article ID 652702, 10 pages doi:10.4061/2011/652702 Research Article Effect of Steric Constraint at the γ-Backbone Position on the Conformations and Hybridization Properties of PNAs Matthew J. Crawford, Srinivas Rapireddy, Raman Bahal, Iulia Sacui, and Danith H. Ly Department of Chemistry, Center for Nucleic Acids Science and Technology (CNAST), Mellon Institute, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA Correspondence should be addressed to Danith H. Ly, dly@ Received 26 August 2010; Revised 6 January 2011; Accepted 14 March 2011 Academic Editor: P. E. Nielsen Copyright © 2011 Matthew J. Crawford et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conformationally preorganized peptide nucleic acids (PNAs) have been synthesized through backbone modiﬁcations at the γ- position, where R = alanine, valine, isoleucine, and phenylalanine side chains. The eﬀects of these side-chains on the conformations and hybridization properties of PNAs were determined using a combination of CD and UV-Vis spectroscopic techniques. Our results show that the γ-position can accommodate varying degrees of sterically hindered side-chains, reaﬃrming the bimodal function of PNAs as the true hybrids of “peptides” and “nucleic acids.” 1. Introduction that are connected through a ﬂexible carboxymethylene linker. Despite the structural departure from the natural Oligonucleotides are becoming increas