effects of stratification and hormone treatments on germination and physio-biochemical properties of taxus chinensis var. mairei seed分层和激素治疗对发芽的影响和physio-biochemical属性的红豆杉var. mairei种子.pdfVIP
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effects of stratification and hormone treatments on germination and physio-biochemical properties of taxus chinensis var. mairei seed分层和激素治疗对发芽的影响和physio-biochemical属性的红豆杉var. mairei种子
American Journal of Plant Sciences, 2012, 3, 829-835 829
/10.4236/ajps.2012.37100 Published Online July 2012 (http://www.SciRP.org/journal/ajps)
Effects of Stratification and Hormone Treatments on
Germination and Physio-Biochemical Properties of
Taxus chinensis var. mairei Seed
1 1 2*
Yanjie Zhang , Shunbao Lu , Handong Gao
1College of Life Science, Jiangxi Normal University, Nanchang, China; 2College of Forest Resources and Environment, Nanjing
Forestry University, Nanjing, China.
*
Email: gaohd@
Received April 27th, 2012; revised May 25th, 2012; accepted June 4th, 2012
ABSTRACT
In order to learn dormancy mechanism of Taxtts chinensis var. mairei seed, effects of stratification and hormone treat-
ments on physiological and biochemical indexes of the seeds after stratifying for eight months were researched. The
content of amylase activity and soluble sugar gradually increased at first stratification, and then to the maximum for the
end of after-ripening period. With the dormancy of seed relieving, some soluble sugar of endosperm was used or con-
sumed by respiration and that led to their content decreased. Seed soluble protein was gradually accumulated at the be-
ginning of stratification and then the initial raw material of protein synthesis of embryo during development had been
used or in part converted into carbohydrates for energy consumption, so that the soluble protein content decreased. The
fat and starch content slowly decreased during stratification, which showed that the storage of material had continually
carrying out catabolism and conversion, which might provide energy sources for seed germination in the stratification
process. The peroxidase activity of Taxus seeds increased gradually at the beginning of stratification, and then reached
maximum
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