effectiveness of bacterial and fungal isolates to control phoma lingam on oilseed rape brassica napus细菌和真菌隔离控制的有效性phoma男性生殖器像在油菜的芸苔属植物显著.pdfVIP

effectiveness of bacterial and fungal isolates to control phoma lingam on oilseed rape brassica napus细菌和真菌隔离控制的有效性phoma男性生殖器像在油菜的芸苔属植物显著.pdf

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effectiveness of bacterial and fungal isolates to control phoma lingam on oilseed rape brassica napus细菌和真菌隔离控制的有效性phoma男性生殖器像在油菜的芸苔属植物显著

American Journal of Plant Sciences, 2012, 3, 773-779 773 /10.4236/ajps.2012.36093 Published Online June 2012 (http://www.SciRP.org/journal/ajps) Effectiveness of Bacterial and Fungal Isolates to Control Phoma lingam on Oilseed Rape Brassica napus 1 2* 3 4 Omar Hammoudi , Mazen Salman , Ruba Abuamsha , Ralf-Udo Ehlers 1Agriculture Scientific Research Center, Lattakia, Syria; 2Palestine Technical University-Kadoorie (PTUK), Tulkarm, Palestine; 3National Agriculture Research Center (NARC), Jenin, Palestine; 4Department of Biotechnology Biological Control, Institute for Phytopathology, Christian-Albrechts-University Kiel, Kiel, Germany. Email: salman_mazen@daad-alumni.de Received February 28th, 2012; revised March 30th, 2012; accepted April 6th, 2012 ABSTRACT Blackleg disease caused by Phoma lingam is an important disease of oil seed rape (Brassica napus) causing losses up to 95%. The efficacy of microbial antagonists against P. lingam in greenhouse was tested. Serratia plymuthica HRO-C48 and Gliocladium catenulatum J1446 were able to reduce the disease intensity of OSR cotelydones infested with P. lin- gam at rates 44% and 52% respectively. The reduction of the infestation of the root collar in BBCH14/15 was evaluated as well. Plants treated with a suspension of the antagonists (2 × 105 cfu/plant) and inoculated with either pycnidiospore suspension (2 × 107 cfu/ml) or agar disks grown with P. lingam mycelium, showed a reduced infestation rate of 53% - 93% in the presence of S. plymuthica and 46% - 77% in the presence of G. catenulatum. The efficacy of the antagonist depends highly on their concentration inside OSR seeds. Below 105 cfu/seed no significant difference was recorded between control u

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