DNA—PKcs在乳腺癌组织中的表达与分期相关性研究.pdfVIP

中瘤预防与治疗 2008年第21卷第1期 · 25· DNA—PKcs在乳腺癌组织中的表达与分期相关性研究术 王晓莉，李文辉△，秦继勇，夏耀雄 (云南省肿瘤医院放疗科，昆明 650118) [摘要] 目的：探讨乳腺癌组织中DNA依赖蛋白激酶(DNA dependent protein kinase，DNA．PK)的催化亚单位 DNA—PKcs的表达变化与分期的相关性。方法：采用免疫组化SP法检测30例I期乳腺癌组织，11例IIIB、IIIC期 乳腺癌组织及19例Ⅳ期乳腺癌组织中DNA．PKcs的表达情况。结果：DNA．PKcs在I期乳腺癌组织中和其癌旁正 常组织中均呈高表达，两者表达具有一致性(P0．05)。DNA．PKcs在IIIB、Ⅲc期和Ⅳ期乳腺癌组织中的表达明显 低于I期乳腺癌(P0．05和P0．01)。同时，把IIIB、IIIC期和Ⅳ期合并后，与I期比较，DNA．PKcs表达差异有极 显著性意义(P0．01)。结论：DNA．PKcs的低表达在乳腺癌发生进展和远处转移中有重要意义，对乳腺癌的分期 有一定指导意义，可能成为乳腺癌分期的生物学指标。 [关键词]乳腺癌；DNA．PKcs；分期；免疫组化 [中图分类号]R737．9；R730．231 [文献标识码]A [文章编号]1674—0904(2008)01—0025—03 A Study on Tissue DNA-PKcs Protein Expression and It’S Relationship with Staging in Breast Cancer WANG Xiao-li．LI Wen—hui，QIN Ji—yong，et a1． (The Radiotherapy Department，Tumor Hospital of Yunnan Province，Kunming 6501 18，China) [Abstract] 0bjective： To investigate the relationship of the expression change of DNA—PKcs in breast cancer tissue with tumol staging．Methods： By using immunohistochemical SP method，the expression of DNA-PKcs was detected in 30 cases stage I breast cancer tissues，1 1 cases of stage III B and stage III C breast cancer tissues，and 19 cases of stage 1V breast cancer tissues respec— tively．Results： There was no statistically significant correlation of DNA-PKcs expression levels with stage I breast cancer tissues and the corresponding normal tissues(P0．05)，and the expression levels of DNA-PKcs in both were high．The expression levels of DNA— PKcs in stage IH B．stage IH C and stage IV breast cancer tissues were lower than those in stage I breast cancer tissues(P0．05 and P 0．01)．The expression of DNA．PKcs between stage I and stage III+IV was obviously different(P0．01)．Conclusion： The low．expression of DNA．PKcs may