repeated doses of uvr cause minor alteration in cytokine serum levels in humans重复剂量的紫外线辐射导致人类小血清细胞因子水平的改变.pdf

Mediators of Inflammation • 2005:5 (2005) 298–303 • PII: S0962935105507014 • DOI: 10.1155/MI.2005.298 RESEARCH COMMUNICATION Repeated Doses of UVR Cause Minor Alteration in Cytokine Serum Levels in Humans Joanna Narbutt,1 Aleksandra Lesiak,1 Malgorzata Skibinska,2 Anna Wozniacka,1 Anna Sysa-Jedrzejowska,1 Joalanta Lukamowicz,3 and Henk van Loveren4 1Department of Dermatology, Medical University of Lodz, Krzemieniecka 5, 94-017 Lodz, Poland 2Department of Dermatology, Basildon University Hospital, SS165NL Essex, England 3Laboratory of Immunochemical Research, Polish Mother’s Memorial Hospital-Research Institute, 93-338 Lodz, Poland 4National Public Health Institute, Bilthoven, 3720 BA, The Netherlands Received 6 April 2005; accepted 6 June 2005 The aim of our study was to compare serum concentration of IL-1β, IL-6, IL-8, IL-10, and TNF-α in 105 healthy volunteers before and after exposure to UVR: 25 subjects (10 days of UVB), 55 (10 days of UVB or solar-simulated radiation, followed by acute UVB dose), and 25 (local high dose of UVB). In all the individuals blood samples were analyzed before and after final irradiation by chemiluminescence assay. After 10 days of UVB irradiation a statistically significant increase in serum concentration only in IL-8 (P .05) and strong tendency in TNF-α (P = .05) were observed. The applied schedules of irradiation have minor impact on serum cytokine level and still a threshold dose of UVR causing systemic immune impairment is unknown. INTRODUCTION important cytokines is TNF-α, secreted under various stimuli suc