免疫抑制剂FTY720对小鼠肝移植瘤抑制作用探究.docVIP

免疫抑制剂FTY720对小鼠肝移植瘤抑制作用探究[摘要] 目的：探讨FTY720的免疫抑制作用及其对小鼠肝移植瘤的抑制作用。方法：小鼠肝癌Hepa1-6细胞按2×106/只的量接种于C57BL/6j小鼠皮下。当C57BL/6j小鼠移植瘤直径至0.30 cm，将40只成瘤小鼠随机分为4组，分别用以下药物灌胃10 d，环孢素组：环孢素10 mg/(kgd)；FTY720组：FTY720 3 mg/(kgd)；5-氟尿嘧啶（5-Fu）组：5-Fu 50 mg/(kgd)；生理盐水组：0.9%氯化钠注射液1 mg/(kgd)。检测胸腺、脾指数、外周血T淋巴细胞计数、肿瘤体积、外周血甲胎蛋白、凋亡指数、瘤体Bcl-2和Caspase-3的表达。结果：环孢素组和FTY720组胸腺、脾指数及T淋巴细胞百分比低于生理盐水组（P＜0.05，P＜0.01）；而5-氟尿嘧啶组与生理盐水组比较，差异无显著性（P＞0.05）；5-氟尿嘧啶组与FTY720组肿瘤体积与甲胎蛋白含量低于生理盐水组（P＜0.05，P＜0.01），凋亡指数高于生理盐水组（P＜0.05，P＜0.01）；而环孢素组与生理盐水组比较，差异无显著性（P＞0.05）；5-氟尿嘧啶组和FTY720组小鼠瘤体Bcl-2的表达低于生理盐水组（P＜0.05），而环孢素组与生理盐水组比较，差异无显著性（P＞0.05）；5-氟尿嘧啶组小鼠瘤体Caspase-3的表达低于生理盐水组（P＜0.01）；而FTY720组和环孢素组与生理盐水组比较，差异无显著性（P＞0.05）。结论：FTY720同时具有免疫抑制作用和抗肿瘤作用，而其抗肿瘤作用与Bcl-2的表达降低有关。 [关键词] 免疫抑制作用；抗肿瘤作用；FTY720；Bcl-2；Caspase-3 [中图分类号] R735.7[文献标识码]A [文章编号]1673-7210（2009）05（a）-021-03 Study on the antitumor activity of the immunosuppressive agent FTY720 to hepatocellular transplanted tumor in mice YANG Haibo1, ZHAO Haoliang2, HE Jiefeng2, XUE Wei1 (1.Shanxi Medical University, Taiyuan 030001, China; 2.The First Hospital of Shanxi Medical University, Taiyuan 030001, China) [Abstract] Objective: To study the immunosuppression and antitumor activity of FTY720 to hepatocellular transplanted tumor in mice. Methods: Mice liver cancer Hepa1-6 cells were collected and injected subcutaneously with a dose of 2×106/mouse into C57BL/6j mice. When the diameter of the hepatocellular transplanted tumor reached 0.30 cm, 40 C57BL/6j mice were randomly divided into four groups, each group was lavaged for 10 days with the following medicine separately, Ciclosporin group: Ciclosporin 10 mg/(kg?d); FTY720 group: FTY720 3 mg/(kg?d); 5-Fu group: 5-Fu 50 mg/(kg?d); NS group: NS 1 mg/(kg?d). Then thymus gland and spleen index, the percentage of peripheral blood T-lymphocyte, gross tumor volume, secretory volume of AFP in serum, apoptosis index as well as the expression of Caspase-3 and Bcl-2 were investigated. Results: Thymus gland and spleen index and the percen