astragalus root and elderberry fruit extracts enhance the ifn-β stimulatory effects of lactobacillus acidophilus in murine-derived dendritic cells黄芪和接骨木果实提取物提高ifn-β刺激嗜酸乳杆菌在murine-derived树突细胞的影响.pdfVIP

Astragalus Root and Elderberry Fruit Extracts Enhance the IFN-b Stimulatory Effects of Lactobacillus acidophilus in Murine-Derived Dendritic Cells 1 1 1 1 2 Hanne Frøkiær *, Louise Henningsen , Stine Broeng Metzdorff , Gudrun Weiss , Marc Roller , 3 3 4 John Flanagan , Emilie Fromentin , Alvin Ibarra 1 Department of Veterinary Disease Biology, Copenhagen University, Frederiksberg, Denmark, 2 Naturex SA, Avignon, France, 3 Naturex Spain, Quart de Poblet, Spain, 4 Naturex Incorporated, South Hackensack, New Jersey, United States of America Abstract Many foods and food components boost the immune system, but little data are available regarding the mechanisms by which they do. Bacterial strains have disparate effects in stimulating the immune system. Indendritic cells, the gram- negative bacteria Escherichia coli upregulates proinflammatory cytokines, whereas gram-positive Lactobacillus acidophilus induces a robust interferon (IFN)-b response. The immune-modulating effects of astragalus root and elderberry fruit extracts were examined in bone marrow-derived murine dendritic cells that were stimulated with L. acidophilus or E. coli. IFN-b and other cytokines were measured by ELISA and RT-PCR. Endocytosis of fluorescence-labeled dextran and L. acidophilus in the presence of elderberry fruit or astragalus root extract was evaluated in dendritic cells. Our results show that both extracts enhanced L. acidophilus-induced IFN-b production and slightly decreased the proinflammatory response to E. coli. The enhanced IFN-b production was associated with upregulation of toll-like receptor 3 and to a varying degree, the cytokines IL-12, IL-6, IL-1b a