cardiac remodeling in fish strategies to maintain heart function during temperature change心脏重塑的鱼在温度变化策略来维持心脏功能.pdfVIP

Cardiac Remodeling in Fish: Strategies to Maintain Heart Function during Temperature Change 1 2 2 3,4 1 Jordan M. Klaiman , Andrew J. Fenna , Holly A. Shiels , Joseph Macri , Todd E. Gillis * 1 Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada, 2 Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom, 3 Department of Laboratory Medicine, Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, Hamilton, Ontario, Canada, 4 Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada Abstract Rainbow trout remain active in waters that seasonally change between 4uC and 20uC. To explore how these fish are able to maintain cardiac function over this temperature range we characterized changes in cardiac morphology, contractile function, and the expression of contractile proteins in trout following acclimation to 4uC (cold), 12uC (control), and 17uC (warm). The relative ventricular mass (RVM) of the cold acclimated male fish was significantly greater than that of males in the control group. In addition, the compact myocardium of the cold acclimated male hearts was thinner compared to controls while the amount of spongy myocardium was found to have increased. Cold acclimation also caused an increase in connective tissue content, as well as muscle bundle area in the spongy myocardium of the male fish. Conversely, warm acclimation of male fish caused an increase in the thickness of the compact myocardium and a decrease in the amount of spongy myocardium. There was also a decrease in connective tissue content in both myocardial layers. In contrast, there was no