characterization and expression of glutamate dehydrogenase in response to acute salinity stress in the chinese mitten crab, eriocheir sinensis表征和谷氨酸脱氢酶的表达,以应对急性盐度压力在中华绒螯蟹,中华绒螯蟹.pdfVIP

Characterization and Expression of Glutamate Dehydrogenase in Response to Acute Salinity Stress in the Chinese Mitten Crab, Eriocheir sinensis 1 1 1 1 2 3 1 Yueru Wang , Erchao Li *, Na Yu , Xiaodan Wang , Chunfang Cai , Boping Tang , Liqiao Chen *, Alain Van Wormhoudt4 1 School of Life Science, East China Normal University, Shanghai, China, 2 School of Basic Medicine and Biological Science, Soochow University, Suzhou, China, 3 Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Basic Medicine and Biological Science, Yancheng Teachers University, ´ Yancheng, China, 4 UMR5178, Station de Biologie Marine du Museum National d’Histoire Naturelle, BP225, Concarneau, France Abstract Background: Glutamate dehydrogenase (GDH) is a key enzyme for the synthesis and catabolism of glutamic acid, proline and alanine, which are important osmolytes in aquatic animals. However, the response of GDH gene expression to salinity alterations has not yet been determined in macro-crustacean species. Methodology/Principal Findings: GDH cDNA was isolated from Eriocheir sinensis. Then, GDH gene expression was analyzed in different tissues from normal crabs and the muscle of crabs following transfer from freshwater (control) directly to water with salinities of 16% and 30%, respectively. Full-length GDH cDNA is 2,349 bp, consisting of a 76 bp 5 9- untranslated region, a 1,695 bp open reading frame encoding 564 amino acids and a 578 bp 39- untranslated region. E. sinensis GDH showed 64–90% identity with protein sequences of mammalian and crustacean species. Muscle was the dominant expression source among