circulating apoptotic progenitor cells in patients with congestive heart failure循环凋亡祖细胞在充血性心力衰竭患者.pdfVIP

Circulating Apoptotic Progenitor Cells in Patients with Congestive Heart Failure . . Dael Geft , Shmuel Schwartzenberg , Ori Rogowsky, Ariel Finkelstein, Jacob Ablin, Sofia Maysel- Auslender, Dov Wexler, Gad Keren, Jacob George* Department of Cardiology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel Abstract Background: Circulating CD34+ endothelial progenitor cells (EPCs) are capable of differentiating into mature endothelial cells to assist in angiogenesis and vasculogenesis. We sought to quantify the numbers of apoptotic progenitors in patients with congestive heart failure. Methods and Results: Peripheral blood mononuclear cells were isolated by Ficoll density-gradient from 58 patients with various degrees of heart failure and 23 matched controls. Apoptosis in progenitor CD34+ cells was assessed using the Annexin V-PE/PI detection kit, and FACS analysis was performed with triple staining for CD34, annexin-V and propidium iodide. The percentage of early and late apoptotic progenitor cells was determined in the subject groups and was correlated with clinical characteristics. While there was no significant difference in total CD34 positive cells or early apoptotic progenitors between control subjects and CHF patients (p = 0.42) or between severe and mild/moderate CHF groups (p = 0.544), there was an elevated number of late apoptotic progenitors in the severe CHF group compared with the mild/moderate CHF group (p = 0.03). Late apoptotic progenitors were significantly increased in CHF patients as compared to matched controls. There was also an inverse correlation between late apoptotic progenitors and ejection fraction (r = 20.252, p = 0.028) as well as a positive association with NYHA class (r