comparative genomics of multidrug resistance-encoding incac plasmids from commensal and pathogenic escherichia coli from multiple animal sources比较基因组学多药性resistance-encoding incac质粒从共生体,从多个动物源致病性大肠杆菌.pdfVIP

Comparative Genomics of Multidrug Resistance- Encoding IncA/C Plasmids from Commensal and Pathogenic Escherichia coli from Multiple Animal Sources ´ ´ 1 1,2 1 Claudia Fernandez-Alarcon , Randall S. Singer , Timothy J. Johnson * 1 Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, United States of America, 2 Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile Abstract Incompatibility group A/C (IncA/C) plasmids have received recent attention for their broad host range and ability to confer resistance to multiple antimicrobial agents. Due to the potential spread of multidrug resistance (MDR) phenotypes from foodborne pathogens to human pathogens, the dissemination of these plasmids represents a public health risk. In this study, four animal-source IncA/C plasmids isolated from Escherichia coli were sequenced and analyzed, including isolates from commercial dairy cows, pigs and turkeys in the U.S. and Chile. These plasmids were initially selected because they either contained the floR and tetA genes encoding for florfenicol and tetracycline resistance, respectively, and/or the blaCMY-2 gene encoding for extended spectrum b-lactamase resistance. Overall, sequence analysis revealed that each of the four plasmids retained a remarkably stable and conserved backbone sequence, with differences observed primarily within their accessory regions, which presumably have evolved via horizontal gene transfer events involving multiple modules. Comparison of these plasmids with other available IncA/C plasmid sequences further defined the core and accessory elements of these