diagnostic accuracy of ns1 elisa and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viraemia and antibody responsesns1 elisa诊断准确性和横向流对登革热的快速检测敏感性,特异性和病毒血症和抗体反应的关系.pdfVIP

Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses 1 1,2 1,2 1 3 Vu Ty Hang , Nguyen Minh Nguyet , Dinh The Trung , Vianney Tricou , Sutee Yoksan , Nguyen Minh 2 2 2 1,4 1,4 1,4 Dung , Tran Van Ngoc , Tran Tinh Hien , Jeremy Farrar , Bridget Wills , Cameron P. Simmons * 1 Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Viet Nam, 2 Hospital for Tropical Diseases, Ho Chi Minh City, Viet Nam, 3 Center for Vaccine Development, Institute of Science and Technology for Research and Development, Mahidol University, Bangkok, Thailand, 4 Centre for Clinical Vaccinology and Tropical Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom Abstract Background: Dengue is a public health problem in many countries. Rapid diagnosis of dengue can assist patient triage and management. Detection of the dengue viral protein, NS1, represents a new approach to dengue diagnosis. Methodology/Principal Findings: The sensitivity and specificity of the Platelia NS1 ELISA assay and an NS1 lateral flow rapid test (LFRT) were compared against a gold standard reference diagnostic algorithm in 138 Vietnamese children and adults. Overall, the Platelia NS1 ELISA was modestly more sensitive (82%) than the NS1 LFRT (72%) in confirmed dengue cases. Both ELISA and LFRT assays were more sensitive for primary than secondary dengue, and for specimens collected within 3 days of illness onset relative to later time points. The pre