dipstick for rapid diagnosis of shigella flexneri 2a in stool2快速诊断试纸的弗氏志贺菌在凳子上.pdfVIP

Dipstick for Rapid Diagnosis of Shigella flexneri 2a in Stool 1 2,3 4 4 2,3 2,3,5 Faridabano Nato , Armelle Phalipon , Lan Phuong Thi Nguyen , Tai The Diep , Philippe Sansonetti , Yves Germani * ´ ´ ´ 1 Plate-Forme 5-Production de Proteines Recombinantes et d’Anticorps, Institut Pasteur, Paris, France, 2 Unite de Pathogenie Microbienne ´ ´ ´ ´ Moleculaire, Institut Pasteur, Paris, France, 3 Unite Institut National de la Sante et de la Recherche Medicale (INSERM) 786, Paris, France, 4 Insitut ´ Pasteur, Ho Chi Minh City, Vietnam, 5 Reseau International, Institut Pasteur, Paris, France Background. Shigellosis or bacillary dysentery, an acute bloody diarrhoea, is a major public health burden in developing countries. In the absence of prompt and appropriate treatment, the infection is often fatal, particularly in young malnourished children. Here, we describe a new diagnostic test for rapid detection, in stool, at the bedside of patients, of Shigella flexneri 2a, the most predominant agent of the endemic form of the disease. Methodology/Principal Findings. The test is based on the detection of S.flexneri 2a lipopolysaccharide (LPS) using serotype 2a-specific monoclonal antibodies coupled to gold particles and displayed on one-step immunochromatographic dipstick. A concentration as low as 20 ng/ml of LPS is detected in distilled water and in reconstituted stools in under 15 minutes. The threshold of detection corresponds to a concentrati