speciation of inorganic selenium and selenoamino acids by an hplc-uv-hg-afs system物种形成的无机硒和selenoamino hplc-uv-hg-afs酸的系统.pdfVIP

Journa l of Automated Methods Management in Chemistry , Vol. 23, No. 6 (November– December 2001 ) pp. 167–172 Speciation of inorganic selenium and selenoamino acids by an HPLC-UV-HG-AFS system 1;2 3 3 an increasing need for selenium speciation methods that I. Ipolyi , W. Corns , P. Stockwell and P. Fodor1 not only the well-supplied research laboratorie s can 1 Department of Applied Chemistry, Szent Istvan University, 29. Villanyi ut, H- aå ord. 1118, Budapest, Hungary; 2 e-mail: iipolyi@omega.kee.hu; 3 PS Analytical Ltd, Arthur House, Unit 3, Cray¢ elds Industrial Estate, Main Road, St Pauls Cray, Analytical systems developed for selenium speciation Orpington, Kent BR5 3HP, UK employ a powerful high-performance liquid chromato- graphy (HPLC) coupled to a speci® c atomic detector For the on-line speciation of selenocystin e (SeCys), selenomethio - with a high eæ ciency sample introduction system. nine (SeMet), selenoethionin e (SeEt), selenite (Se(IV)) and selenate (Se(VI)), a high-performanc e liquid chromatography - Fast and eæ cient separation of the inorganic from the UV irradiation-hydride generation-atomi c £ uorescence spectro - organic selenium species can be solved microcolumns [7]. metric method is described . Separation was carried out on a Diå erent selenoamino acids and the inorgani c forms conventional reversed-phase C18 column modi¢ ed with didodecyl - (selenite and selenate) can be separated on cation-ex- dimethylammonium bromide with gradien t elution applying two change, str