spectrophotometric determination of mycophenolate mofetil as its charge-transfer complexes with two -acceptors光度法测定霉酚酸酯作为其电荷转移复合物有两个受体.pdfVIP

Hindawi Publishing Corporation Journal of Analytical Methods in Chemistry Volume 2012, Article ID 875942, 8 pages doi:10.1155/2012/875942 Research Article Spectrophotometric Determination of Mycophenolate Mofetil as Its Charge-Transfer Complexes with Two π-Acceptors K. B. Vinay, H. D. Revanasiddappa, M. S. Raghu, Sameer. A. M. Abdulrahman, and N. Rajendraprasad Department of Chemistry, University of Mysore, Manasagangotri, Mysore 570 006, India Correspondence should be addressed to H. D. Revanasiddappa, hdrevanasiddappa@ Received 10 November 2011; Revised 15 January 2012; Accepted 20 January 2012 Academic Editor: Ricardo Jorgensen Cassella Copyright © 2012 K. B. Vinay et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Two simple, selective, and rapid spectrophotometric methods are described for the determination of mycophenolate mofetil (MPM) in pure form and in tablets. Both methods are based on charge-transfer complexation reaction of MPM with p-chloranilic acid (p-CA) or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) in dioxane-acetonitrile medium resulting in coloured product measurable at 520 nm (p-CA) or 580 nm (DDQ). Beer’s law is obeyed over the concentration ranges of 40–400 and 12–120 μg mL−1 MPM for p-CA and DDQ, respectively, with correlation coefficients (r) of 0.9995 and 0.9947. The apparent molar absorptivity 3 3 −1 −1 values are calculated to be 1.06 × 10 and 3.87 × 10 L mol cm , respectively, and the corresponding Sandell’s sensitivities are 0.4106 and 0.1119 μg cm−1. The limits of detection (LOD) and qua