静脉注射acarbose对大鼠胰岛素和胰升糖素分泌的影响（Effects of intravenous injection of acarbose on insulin and glucagon secretion in rats）.docVIP

静脉注射acarbose对大鼠胰岛素和胰升糖素分泌的影响（Effects of intravenous injection of acarbose on insulin and glucagon secretion in rats） Effects of intravenous injection of Acarbose on insulin and glucagon secretion in rats 2010-03-25 Tian Hui bucket Tian Ge Ge successfully send wild Hao Acarbose (Acarbose) for clinical treatment of diabetes for many years, that the drug in reducing blood glucose at the same time, also lower blood insulin levels (INS). This has been previously considered because Acarbose lowers blood sugar, thereby reducing hyperglycemia and stimulating beta cell secretion of INS. The recent report of intestinal type Acarbose can directly inhibit rat islet lysosomal enzyme activity in 1, 2, thereby inhibiting glucose induced INS secretion can be inhibited by the sulfonylurea (SU) induced INS release. It is assumed that Acarbose has other hypoglycemic mechanisms in addition to the inhibition of gut alpha glucosidase. This study investigated the effects of intravenous Acarbose on glucose regulation and the secretion of INS and glucagon in rats. Materials and methods 1. materials: 8 week old male Wistar rats, weighing between 250 ~ 350 grams. Were randomly divided into three groups: (1) normal rats glucose load group (G group); (2) normal rat insulin hypoglycemia group (INS group); (3) streptozotocin diabetic rats (STZ-DM group), the rats were injected with STZ three days after the blood glucose level greater than or equal to 16.7mmol/L. A week later, for testing, before the test without INS treatment. 2. methods: fasting 10 hours. During the test, blood was collected from the internal jugular vein after anesthesia and heparin anticoagulation in the tube. The following test group and methods: (1) G group: 12 rats, 6 rats as the control group (G-C group), the other 6 for the application of Acarbose (G-A group), 0.6 g/kg (3.3mmol/kg) according to the dose of intravenous injection of glucose, glucose in -15 before injection, 0 points and 10 points after injection. 30 points,