高效液相色谱法测定依诺沙星胶囊的含量（Determination of the contents of Enoxacin Capsuless high performance liquid chromatography）.docVIP

高效液相色谱法测定依诺沙星胶囊的含量（Determination of the contents of Enoxacin Capsuless high performance liquid chromatography） Determination of the contents of Enoxacin Capsuless high performance liquid chromatography Update Date: 2011-08-14, Huang Xiu, Hong Xiu, Chen Shui Zhuan Abstract: in this paper, the content was determined by HPLC at Enoxacin Capsules. Mobile phase: -0.05 Mol*L1 acetonitrile citric acid (20: 80), the detection wavelength was 266 nm, the average recovery was 100.6% (n = 9, RSD = 1%). Keywords: high performance liquid chromatography; Enoxacin Determination, of, Enoxacin, Capsules, by, HPLC HUANG, Rong-zhen, XIU, Hong, CHEN, Shui-zhuang (Fujian, Institute, for, Drug, Control) ABSTRACT:This paper reported the determination of Enoxacin Capsules by HPLC.Analysis was performed on a Novapak-C18 (with acetonitrile-0.05 150 mm 3.9 apparatus) mol / L citric acid (20:80) as the mobile phase and detection at 266 nm.The average recovery was 100.6% (n = 9) and RSD was 1%. KEY WORDS:HPLC; Enoxacin Enoxacin is a fluoroquinolone antibiotic, first marketed in Japan in 1986. Our country was successfully developed and put into production in 1989. Enoxacin (ENX) wide antimicrobial spectrum, high concentration, blood and tissue metabolism, the in vivo antibacterial activity of norfloxacin was 2 ~ 9 times, so in the world to get promotion and use. The Pharmacopoeia of Chinese Pharmacopoeia 2000 has been included, and the content analysis method is absorption coefficient method. The content of this paper, the determination of enoxacin gum by HPLC method is accurate, easy to operate. 1 instruments and reagents 1.1 instrument high performance liquid chromatography Waters series. 1.2 reagents, samples and reference products are supplied by Fujian southeast pharmaceutical factory. The sodium hydroxide and citric acid are pure and acetonitrile is chromatographic pure and ultra pure water. 2 chromatographic conditions Chromatographic column: Novapak-C18 (3.9 * 150 mm), mobile phase: aceto