PDCD4在人脑胶质瘤中的表达及恶性程度的相关研究.pdfVIP

内 容 摘 要 目的：人脑胶质瘤是颅内最常见的恶性肿瘤，尽管新的诊疗技术不断出现，但其 治疗效果不佳，生存率没有明显提高。因此，从分子水平上探讨胶质瘤的生物行为显 得尤为重要。PDCD4(programed cell death 4)是近年来新发现的抑癌基因，能够通过抑 制蛋白转录和翻译过程抑制肿瘤细胞的生长。探讨新的抑癌基因PDCD4 的生物学行 为与肿瘤恶性程度的关系，为临床医师准确判断肿瘤的恶性程度提供新的依据，为肿 瘤分子靶向治疗以及新的抗癌药物开发提供理论和实验基础。 方法：（1）应用免疫组化S-P 法检测64 例人胶质瘤组织切片和 8 例冷冻保存的 正常脑组织中PDCD4 的表达水平，探讨其与肿瘤的分化程度和临床病理参数的关系。 （2 ）应用RT-PCR 检测PDCD4 在不同病理级别的胶质瘤基因转录水平，探讨其与肿 瘤的恶性程度和临床病理参数的关系。 结果：胶质瘤中的PDCD4 蛋白水平和基因转录水平明显下调；PDCD4 蛋白水平 和基因转录水平与肿瘤的病理分级密切相关, 在低恶性胶质瘤中呈高表达，随着恶性 程度上升，表达明显下调。 结论：PDCD4 表达与人脑胶质瘤的恶性程度呈负相关，检测PDCD4 表达水平有 助于评价肿瘤的生物学行为，可为针对肿瘤的分子靶向治疗提供参考依据。 关键词：胶质瘤； PDCD4 ； 生物学行为 II Abstract Objective Human glioma is the most of common tumor that arises in the central nervous system. The major clinical therapy methods are operation, radiotherapy and chemical therapy. Therapeutic effect and survival rate of glioma are negative. Therefore, studies of the molecular biology of the tumor are key issues to getting insight into the mechanisms underlying the pathogenesis of glioma. PDCD4(programmed cell death 4) is a new cell-apoptosis related gene. The research human tumor has been recently shown that it may be a new tumor suppressor gene and play an important role in tumor generation and development. It could inhibit the growth of tumor cells by inhibition of the gene transcription and translation. Probing the expression of PDCD4 will be helpful to evaluate the biological behaviors of glioma and provide the theoretical and experimental reference for the molecule target treatment and anticancer drugs of tumors. Methods (1)To examine the relationship between PDCD4 expression and clinical pathological characteristics by immunohistoehemistry