about viral hepatitis nitric oxide and its clinical significance（关于病毒性肝炎一氧化氮及其临床意义）.docVIP

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about viral hepatitis nitric oxide and its clinical significance（关于病毒性肝炎一氧化氮及其临床意义）.doc

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about viral hepatitis nitric oxide and its clinical significance（关于病毒性肝炎一氧化氮及其临床意义）

About Viral Hepatitis nitric oxide and its clinical significance Author: Liu Shulin Gu rights Yang Qiurong [Keywords] viral hepatitis Keywords: viral hepatitis; nitric oxide; alanine aminotransferase 0 Introduction Nitric oxide (NO awareness in recent years, a new kind of an important carrier of information exchange between the cells, both second messenger and neurotransmitter function, is widely involved in the regulation of physiological functions and pathological processes occur. Nitric oxide viral hepatitis relationship between the two has caused widespread concern in the medical profession, to this end, the experimental determination of the nitric oxide content of the serum of patients with viral hepatitis, and to explore its clinical significance in viral hepatitis. 1 Materials and methods 1.1 Selection of cases were all Tangdu hospital inpatients 87 (62 males and 25 females, aged 10 to 63 years, an average of 31 years old .87 inpatients, including 37 cases of acute jaundice hepatitis, 38 cases of chronic hepatitis, severe hepatitis, 12 patients. diagnosis all in compliance with the the 1995-05 national viral hepatitis Conference in Beijing revised diagnostic criteria. Normal control group, 30 (18 males and 12 females, aged 22 to 47 years old, 33 years old on average. Both healthy subjects. 1.2 Detection 1.2.1 Specimen Collection fasting venous blood collection 2mL within 2 hours the conventional separation of serum ALT, the day, the remaining serum stored at -20 ℃ under test. 1.2.2 Nitric Oxide Determination of endogenous NO half-life is extremely short, it is difficult to directly detect NO can rapidly oxidized to stable metabolites NO2-and NO3-oxidation products, the latter two biologically inactive, serum NO2-/ NO3 - The level has become one of the indirect effective indicators reflect the content of NO in vivo the experiment using the indirect detection of NO content of the chemical colorimetric detection of serum nitr