adult bone marrow stromal cells cultured in vitro and induced to differentiate into osteoblasts（成人骨髓基质细胞体外培养和诱导分化成成骨细胞）.docVIP

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adult bone marrow stromal cells cultured in vitro and induced to differentiate into osteoblasts（成人骨髓基质细胞体外培养和诱导分化成成骨细胞）.doc

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adult bone marrow stromal cells cultured in vitro and induced to differentiate into osteoblasts（成人骨髓基质细胞体外培养和诱导分化成成骨细胞）

Adult bone marrow stromal cells cultured in vitro and induced to differentiate into osteoblasts : Teng Yong, HU Yun, An Shujie, Daixian Wen, Zhao Li Bai Jianping, LI Xusheng Lugeon Keywords:] stem cells [Abstract] AIM: To build a method to culture bone marrowderived mesenchymal stem cells (MSCs) in vitro and to investigate the feasibility of inducing MSCs into osteoblasts. METHODS: Human of MSCs were isolated from adult bone marrow and purified by by the percoll were expanded for density gradient centrifugation and cultured in vitro. The MSCs attachment formed after 7-10 d and the MSCs of the passage 3 were chosen to induce into osteoblasts. Fifteen days later, the alkaline phosphatase assay was conducted using modified calciumcobalt staining method, type Ⅰ collagen and osteocalcin assay was conducted using immunohistochemistry and calcium node assay was done using alizarin red staining. RESULTS: Homogeneous MSCs were obtained by percoll density gradient centrifugation. The induced MSCs had typical appearance of osteoblasts. The rate of ALP expression was 81%, the expressions of collagen type Ⅰ and osteocalcin were positive, and calcium nodes were seen. CONCLUSION: The MSCs can be separated from adult bone marrow and cultured in vitro, which can be induced into osteoblasts. [Keywords] bone marrow; stem cells; adult; tissue engineering; osteogenesis / drug effects [Abstract] Objective: To establish adult bone marrow stromal stem cells (MSCs) in vitro, and explore the orientation induced differentiation as osteoblasts of ways. Methods: extract adult bone marrow Percoll density gradient centrifugation method vitro culture stickers wall cell passaging 3rd generation of cells in the culture medium into bone inducer dexamethasone, β glycerophosphate, vitamin C and cultured for 15 d in the inverted microscope cell morphology, calcium - cobalt staining detection of alkaline phosphatase (ALP expression immunohistochemistry (SABC assay of collagen type I, ost