a review on direct electrochemistry of catalase for electrochemical sensors回顾对过氧化氢酶的直接电化学的电化学传感器.pdfVIP

Sensors 2009, 9, 1821-1844; doi:10.3390/ OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review A Review on Direct Electrochemistry of Catalase for Electrochemical Sensors Periasamy Arun Prakash, Umasankar Yogeswaran and Shen-Ming Chen* Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road , Taipei 106, Taiwan (ROC); E-Mails: arunprakash.p@ (P.A.P.); uyogesh@ (U.Y.) * Author to whom correspondence should be addressed; E-Mail: smchen78@; Tel: +886 2270 17147; Fax: +886 2270 25238 Received: 20 January 2009; in revised version: 3 March 2009 / Accepted: 13 March 2009 / Published: 13 March 2009 Abstract: Catalase (CAT) is a heme enzyme with a Fe(III/II) prosthetic group at its redox centre. CAT is present in almost all aerobic living organisms, where it catalyzes the disproportionation of H O into oxygen and water without forming free radicals. In order 2 2 to study this catalytic mechanism in detail, the direct electrochemistry of CAT has been investigated at various modified electrode surfaces with and without nanomaterials. The results show that CAT immobilized on nanomaterial modified electrodes shows excellent catalytic activity, high sensitivity and the lowest detection limit for H O determination. In 2 2 the presence of nanomaterials, the direct electron transfe