a platform for combined dna and protein microarrays based on total internal reflection fluorescence一个结合dna和蛋白质微阵列平台基于全内反射荧光.pdfVIP

Sensors 2012, 12, 1800-1815; doi:10.3390/s120201800 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article A Platform for Combined DNA and Protein Microarrays Based on Total Internal Reflection Fluorescence Alexander Asanov 1,*, Angélica Zepeda 2,* and Luis Vaca 3 1 TIRF Technologies, 951 Aviation Parkway, Suite 700, Morrisville, NC 27560, USA 2 Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, DF 04510, México 3 Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, DF 04510, México; E-Mail: lvaca@ifc.unam.mx * Authors to whom correspondence should be addressed; E-Mails: asn@ (A.A.); azepeda@biomedicas.unam.mx (A.Z.); Tel.: +525-5622-9215 (A.Z.); Fax: +525-5622-9182 (A.Z.). Received: 9 December 2011; in revised form: 13 January 2012 / Accepted: 2 February 2012 / Published: 9 February 2012 Abstract: We have developed a novel microarray technology based on total internal reflection fluorescence (TIRF) in combination with DNA and protein bioassays immobilized at the TIRF surface. Unlike conventional microarrays that exhibit reduced signal-to-background ratio, require several stages of incubation, rinsing and stringency control, and measure only end-point results, our TIRF microarray technology provides several orders of magnitude better signal-to-background ratio, performs analysis rapidly in one step, and measures th