high concentration of oxygen inhibition of rabbit arterial smooth muscle cells proliferation（高浓度的氧气抑制兔动脉平滑肌细胞增殖）.docVIP

High concentration of oxygen inhibition of rabbit arterial smooth muscle cells proliferation Author: Lvan Lin singing Guoliang Wang Xiaoyan [Keywords] oxygen Keywords: oxygen; arterial smooth muscle cells; coronary restenosis Abstract: Objective To investigate the inhibition of hypoxia and high oxygen environment rabbit aortic smooth muscle cell proliferation, indirect judgment hypoxia and coronary restenosis relations and oxygen therapy for PTCA stent coronary restenosis. RPMI1640 culture with calf serum containing 100mL · L-1 the liquid cultured aortic smooth muscle cells of New Zealand white rabbits to 3 to 7 generations calf serum containing 50mL · L-1, RPMI1640 medium arterial smooth muscle cells suspension 100μL joined cultured in 96-well plates, 100,210,250,500 and 750mL · L-1 O2 and pure oxygen environment in accordance with the experimental groups were cultured aortic smooth muscle cells 48h using the MTT assay and 3 H-TdR incorporation. detection of arterial smooth muscle cell proliferation in the number of results to 210mL ?L-1 O2 environment, the number of arterial smooth muscle cell proliferation as a reference control group, the other groups as the experimental group .500 mL ?L-1 O2 to pure oxygen environment of arterial smooth muscle cells proliferation was significantly lower than that of the control group (P lt;0.05,250 mL ?L-1 O2 aortic smooth muscle cell proliferation and 210mL ?L-1 O2 environment no significant difference (Pgt; 0.05,100 mL ?L-1 O2 environment artery The proliferation of the smooth muscle cells was significantly higher than that of the control group (P lt;0.05). conclusion proliferation of arterial smooth muscle cells increased as the culture of the oxygen concentration in the environment, hypoxia promote the proliferation of arterial smooth muscle cells, and high oxygen proliferation of arterial smooth muscle cells significant inhibition; oxygen therapy may help reduce coronary restenosis rate, providing a theoret