hplc method for the determination of the content of isoniazid injection（高效液相色谱方法测定异烟肼注射的内容）.docVIP

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2017-09-06 发布于浙江
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hplc method for the determination of the content of isoniazid injection（高效液相色谱方法测定异烟肼注射的内容）.doc

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hplc method for the determination of the content of isoniazid injection（高效液相色谱方法测定异烟肼注射的内容）

HPLC method for the determination of the content of isoniazid injection [Abstract] Objective To establish a HPLC method for the determination of Isoniazid Injection content. Methods HPLC method using a Hypersil BDS C18 column with acetonitrile 0.075 mol ?L-1 sodium dihydrogen phosphate solution (5:95 as the mobile phase a flow rate of 1.0 mL · min-1, the detection wavelength was 262 nm. isoniazid results in a linear relationship with the peak area in the concentration range of 9.90 ~ 99.00 μg · mL-1 well (r = 0.999 9, the average recovery was 100.3% ( RSD = 1.0%. CONCLUSION The method is simple, fast and accurate, repeatable, as the quality control of this product. [Keywords] HPLC method, isoniazid injection, isoniazid Abstract: Objective To establish an HPLC method for determining the content of isoniazid in isoniazid injection.Method Samples were analyzed on an Hypersil BDS C18 column using acetonitrile 0.075 mol ?L-1 sodium dihydrogen phosphate (5:95) as mobile phase. The flow rate was 1.0 mL ?min-1 and the detection wavelength 262 nm.Results There was a good linearity for isoniazid in the range of 9.90-99.00 μg · mL-1 (r = 0.9999). The average recovery was 100.3% with RSD 1.0 %. Conclusion The method is simple, rapid and accurate, which is suitable for the quality control of isoniazid injection. Keywords:: HPLC; isoniazid injection; isoniazid Isoniazid injection of anti-TB drugs, the Ministry of Health Drug titration with standard potassium bromate the isoniazid content [1]. The article refers to the literature [2] to establish a HPLC method for determination of the content of isoniazid injection, the test results show that the method is simple, rapid, accurate, reproducible and can be used for the Determination of isoniazid injection. 1 instrument and reagent 1.1 Instruments Agilent 1100 liquid chromatograph (Agilent Hypersil BDS C18 column (Phenomenex, the TU 1901 type UV spectrophotometer (Beijing Purkinje General Instrument C