factors affecting the quality of apheresis platelets（影响因素apheresis血小板的质量）.docVIP

Factors affecting the quality of apheresis platelets Network to write papers: Author: Dai Yuee FORCES Xia Aijun [Keywords] blood cells Keywords: blood cells; separator; platelets; impact factors 1 Materials and methods 1.1 materials with reference to the Ministry of Health issued the lt;lt; reference standard physical examination of blood donationgt; select body mass greater than 50kg, platelets greater than 175 ?109 ?L-1 donor and apheresis before 72h not taking aspirin drugs. COBE SpectraTM blood cell separation machine and dedicated apheresis pipeline (United States cobe Company production K-1000 fully automated blood cell analysis instrument (Japan East Asia Company production count for those before harvest, after platelet number and product of red blood cells mixed with the amount of platelet products white blood cell count Nageotte counting plate count of white blood cells, sampling and leukocyte diluent 1:4 mixed charge plate stand for 15min, 200 times under a microscope to count white blood cells. 1.2 apheresis selection of double needle standard procedures, data required input donors, bilateral elbow venipuncture whole blood flow velocity of 30 to 50 mL ?min-1, ACD-A anticoagulant whole blood ratio of 1:8 . 2 Results Donor platelet apheresis platelets (Table 1. Table 1 donor platelet count and apheresis platelets results slightly 3 Discussion Apheresis former donor peripheral platelet count is one of the main factors affect the quality of platelets collected platelet collection volumegt; = 3.0 ?1011, in the case of certain major technical parameters apheresis donors before PLT level of proportional to the amount of platelet collection, but the PLTgt; = 300 ?109 ?L-1, the actual collection is not presented directly proportional to increased and we believe that: ① apheresis donors before PLTgt; 175 × 109 · L-1, and platelet distribution good acquisition effect diagram showed a single peak; leukocyte contamination ② products