原创力文档regulation of c3a receptor signaling in human mast cells by g protein coupled receptor kinases监管c3a受体信号在人类肥大细胞通过g蛋白偶联受体激酶.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
regulation of c3a receptor signaling in human mast cells by g protein coupled receptor kinases监管c3a受体信号在人类肥大细胞通过g蛋白偶联受体激酶
Regulation of C3a Receptor Signaling in Human Mast
Cells by G Protein Coupled Receptor Kinases
Qiang Guo, Hariharan Subramanian, Kshitij Gupta, Hydar Ali*
Department of Pathology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
Abstract
Background: The complement component C3a activates human mast cells via its cell surface G protein coupled receptor
(GPCR) C3aR. For most GPCRs, agonist-induced receptor phosphorylation leads to receptor desensitization, internalization
as well as activation of downstream signaling pathways such as ERK1/2 phosphorylation. Previous studies in transfected
COS cells overexpressing G protein coupled receptor kinases (GRKs) demonstrated that GRK2, GRK3, GRK5 and GRK6
participate in agonist-induced C3aR phosphorylation. However, the roles of these GRKs on the regulation of C3aR signaling
and mediator release in human mast cells remain unknown.
Methodology/Principal Findings: We utilized lentivirus short hairpin (sh)RNA to stably knockdown the expression of GRK2,
GRK3, GRK5 and GRK6 in human mast cell lines, HMC-1 and LAD2, that endogenously express C3aR. Silencing GRK2 or GRK3
expression caused a more sustained Ca2+ mobilization, attenuated C3aR desensitization, and enhanced degranulation as
well as ERK1/2 phosphorylation when compared to shRNA control cells. By contrast, GRK5 or GRK6 knockdown had no
effect on C3aR desensitization, but caused a significant decrease in C3a-induced mast cell degranulation. Interestingly, GRK5
or GRK6 knockdown rendered mast cells more responsive to C3a for ERK1/2 phosphorylation.
Conclusion/Significance: This study demonstrates that GRK2 and GRK3 are involved in C3aR desensitization. Furthermore, it
reveals the novel finding that GRK5 and GRK6 promote C3a-induced mast cell degranulat
您可能关注的文档
- recruitment kinetics of dna repair proteins mdc1 and rad52 but not 53bp1 depend on damage complexity招聘dna修复蛋白mdc1动力学和rad52但不是53 bp1取决于损伤的复杂性.pdf
- recruitment of activation receptors at inhibitory nk cell immune synapses招聘的激活受体抑制nk细胞免疫突触.pdf
- recruitment of both the mirror and the mentalizing networks when observing social interactions depicted by point-lights a neuroimaging study招聘的镜子和mentalizing网络当观察社会交互描述点光源神经成像研究.pdf
- recruited cells can become transformed and overtake pdgf-induced murine gliomas in vivo during tumor progression招募了细胞可以成为转化和超越pdgf-induced小鼠神经胶质瘤体内肿瘤恶化.pdf
- recruitment of occipital cortex during sensory substitution training linked to subjective experience of seeing in people with blindness招聘的枕叶皮质区在感官替代训练与主观经验的人的失明.pdf
- recruitment of cln3 cyclin to promoters controls cell cycle entry via histone deacetylase and other targets招聘cln3细胞周期蛋白启动子控制细胞周期进入通过组蛋白脱乙酰酶和其他目标.pdf
- recruitment and activation of pancreatic stellate cells from the bone marrow in pancreatic cancer a model of tumor-host interaction招聘和胰腺星状细胞激活的骨髓tumor-host胰腺癌模型的交互.pdf
- recurrent activity in higher order, modality non-specific brain regions a granger causality analysis of autobiographic memory retrieval在高阶经常性活动,形态非特异性脑区自传记忆提取的格兰杰因果分析.pdf
- rectangular coordination polymer nanoplates large-scale, rapid synthesis and their application as a fluorescent sensing platform for dna detection矩形配位聚合物nanoplates大规模、快速的合成及其应用程序作为荧光传感平台进行dna检测.pdf
- recovering protein-protein and domain-domain interactions from aggregation of ip-ms proteomics of coregulator complexes恢复蛋白质的聚合和domain-domain交互ip-ms coregulator复合物的蛋白质组学.pdf
- regulation of energy stores and feeding by neuronal and peripheral creb activity in drosophila监管的能量存储和喂养果蝇的神经和周围分子活动.pdf
- regulation of bestrophins by ca2+ a theoretical and experimental study监管bestrophins ca2 +的理论和实验研究.pdf
- regulation of expression of citrate synthase by the retinoic acid receptor-related orphan receptor α (rorα)柠檬酸合成酶的表达调节视黄酸受体相关孤儿受体α(rorα).pdf
- regulation of epidermal growth factor receptor signaling and erlotinib sensitivity in head and neck cancer cells by mir-7抗表皮生长因子受体信号调节和埃罗替尼mir-7敏感性在头部和颈部癌症细胞.pdf
- regulation of gene expression by pi3k in mouse growth plate chondrocytes调节基因表达的pi3k在小鼠生长板软骨细胞.pdf
- regulation of gabaa and glutamate receptor expression, synaptic facilitation and long-term potentiation in the hippocampus of prion mutant micegabaa和谷氨酸受体表达,调节突触便利化和长期势差朊病毒突变小鼠的海马.pdf
- regulation of fibre contraction in a rat model of myocardial ischemia调节纤维收缩心肌缺血大鼠模型.pdf
- regulation of caenorhabditis elegans p53cep-1–dependent germ cell apoptosis by rasmapk signaling监管rasmapk秀丽隐杆线虫p53cep-1-dependent生殖细胞凋亡的信号.pdf
- regulation of gene expression with thyroid hormone in rats with myocardial infarction调节基因表达与甲状腺激素与心肌梗死大鼠.pdf
- regulation of hif-1α and vegf by mir-20b tunes tumor cells to adapt to the alteration of oxygen concentration监管hif-1α和vegf mir-20b曲调肿瘤细胞适应氧浓度的改变.pdf
文档评论（0）