regulation of petrobactin and bacillibactin biosynthesis in bacillus anthracis under iron and oxygen variation监管petrobactin和bacillibactin生物合成在炭疽杆菌铁和氧变化.pdfVIP

Regulation of Petrobactin and Bacillibactin Biosynthesis in Bacillus anthracis under Iron and Oxygen Variation 1 .¤a 2.¤b 2 1 Jung Yeop Lee * , Karla D. Passalacqua , Philip C. Hanna , David H. Sherman * 1 Life Sciences Institute and Departments of Medicinal Chemistry, Chemistry, Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, United States of America, 2 Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America Abstract Background: Bacillus anthracis produces two catecholate siderophores, petrobactin and bacillibactin, under iron-limited conditions. Here, we investigate how variable iron and oxygen concentrations influence the biosynthetic output of both siderophores in B. anthracis. In addition, we describe the differential levels of transcription of select genes within the B. anthracis siderophore biosynthetic operons that are responsible for synthesis of petrobactin and bacillibactin, during variable growth conditions. Methodology/Principal Findings: Accumulation of bacillibactin in B. anthracis Sterne (34F ) and in a mutant lacking the 2 major superoxide dismutase (DsodA1) was almost completely repressed by the addition of 20 mM of iron. In contrast, petrobactin synthesis in both strains continued up to 20 mM of iron. Accumulation of petrobactin and bacillibactin showed a slight increase with addition of low levels of paraquat-induced oxidative stress in wild type B. anthracis Sterne. Cultures grown with high aeration resulted in greater accumulation of petrobactin relative