screening for c3 deficiency in newborns using microarrays新生儿筛查c3缺乏使用微阵列.pdfVIP

Screening for C3 Deficiency in Newborns Using Microarrays 1 ¨ 2 2 ¨ 3 ¨ 4 5 Magdalena Janzi , Ronald Sjoberg , Jinghong Wan , Bjorn Fischler , Ulrika von Dobeln , Lourdes Isaac , 2 ¨ 1 Peter Nilsson *, Lennart Hammarstrom * 1 Division of Clinical Immunology, Karolinska Institutet, KUS Huddinge, Stockholm, Sweden, 2 Department of Proteomics, School of Biotechnology, AlbaNova University Center, KTH-Royal Institute of Technology, Stockholm, Sweden, 3 Children’s Hospital, KUS Huddinge, Stockholm, Sweden, 4 Centre for Inherited Metabolic Diseases, ˜ Karolinska Institutet, KUS Huddinge, Stockholm, Sweden, 5 Laboratory of Complement, Department of Immunology, Institute of Biomedical Sciences, University of Sao ˜ Paulo, Sao Paulo, Brazil Abstract Background: Dried blood spot samples (DBSS) from newborns are widely used in neonatal screening for selected metabolic diseases and diagnostic possibilities for additional disorders are continuously being evaluated. Primary immunodeficiency disorders comprise a group of more than one hundred diseases, several of which are fatal early in life. Yet, a majority of the patients are not diagnosed due to lack of high-throughput screening methods. Methodology/Principal Findings: We have previously developed a system using reverse phase protein microarrays for analysis of IgA levels in serum samples. In this study, we extended the applicability of the method to include determination of complement component C3 levels in eluates from DBSS collected at bir