screening a peptide library by dsc and saxd comparison with the biological function of the parent proteins筛选肽库通过dsc和saxd与母公司的生物功能的蛋白质.pdfVIP

Screening a Peptide Library by DSC and SAXD: Comparison with the Biological Function of the Parent Proteins ´ ´ 1 2 2 ´ ´ 1 Ana J. Perez-Berna , George Pabst , Peter Laggner , Jose Villalaın * ´ ´ 1 Instituto de Biologıa Molecular y Celular, Universidad ‘‘Miguel Hernandez’’, Alicante, Spain, 2 Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences, Graz, Austria Abstract We have recently identified the membranotropic regions of the hepatitis C virus proteins E1, E2, core and p7 proteins by observing the effect of protein-derived peptide libraries on model membrane integrity. We have studied in this work the ability of selected sequences of these proteins to modulate the L -L and L -H phospholipid phase transitions as well as b a a II check the viability of using both DSC and SAXD to screen a protein-derived peptide library. We demonstrate that it is feasible to screen a library of peptides corresponding to one or several proteins by both SAXD and DSC. This methodological combination should allow the identification of essential regions of membrane-interacting proteins which might be implicated in the molecular mechanism of membrane fusion and/or budding. ´ ´ ´ Citation: Perez-Berna AJ, Pabst G, Laggner P, Villalaın J (2009) Screening a Peptide Library by DSC and SAXD: Comparison with the Biological Function of the Parent Proteins. PLoS ONE 4(2): e4356. doi:10.1371/journal.pone.0004356 Editor: Robert J. Geraghty,