sensitive and specific detection of trypanosoma cruzi dna in clinical specimens using a multi-target real-time pcr approach敏感和特定鲁兹锥体dna检测在临床标本使用多目标实时pcr方法.pdfVIP

Sensitive and Specific Detection of Trypanosoma cruzi DNA in Clinical Specimens Using a Multi-Target Real- Time PCR Approach 1 2 3 3 1 Yvonne Qvarnstrom , Alejandro G. Schijman , Vincent Veron , Christine Aznar , Francis Steurer , Alexandre J. da Silva1* 1 Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America, 2 LabMeCh, ´ ´ ´ INGEBI-CONICET, Buenos Aires, Argentina, 3 Laboratoire hospitalier et Universitaire-CH Andree Rosemon, Faculte de Medecine H. Bastaraud-EA 3593, Cayenne, Guyane Franc¸aise Abstract Background: The laboratory diagnosis of Chagas disease is challenging because the usefulness of different diagnostic tests will depend on the stage of the disease. Serology is the preferred method for patients in the chronic phase, whereas PCR can be successfully used to diagnose acute and congenital cases. Here we present data using a combination of three TaqMan PCR assays to detect T. cruzi DNA in clinical specimens. Methods/Principal Findings: Included in the analysis were DNA extracted from 320 EDTA blood specimens, 18 heart tissue specimens, 6 umbilical cord blood specimens, 2 skin tissue specimens and 3 CSF specimens. For the blood specimens both whole blood and buffy coat fraction were analyzed. The specimens were from patients living in the USA, with suspected exposure to T. cruzi through organ transplantation, contact with triatomine bugs or laboratory accidents, and from immunosuppressed patients with suspected Chagas disease reactivation. Real-time PCR was successfully used