原创力文档silencing relaxin-3 in nucleus incertus of adult rodents a viral vector-based approach to investigate neuropeptide function沉默的原子核incertus relaxin-3成年啮齿动物病毒研究神经肽功能的基于矢量的方法.pdfVIP
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silencing relaxin-3 in nucleus incertus of adult rodents a viral vector-based approach to investigate neuropeptide function沉默的原子核incertus relaxin-3成年啮齿动物病毒研究神经肽功能的基于矢量的方法
Silencing Relaxin-3 in Nucleus Incertus of Adult Rodents:
A Viral Vector-based Approach to Investigate
Neuropeptide Function
1,2 2,3 1,2 2 2,4
Gabrielle E. Callander *, Sherie Ma , Despina E. Ganella , Verena C. Wimmer , Andrew L. Gundlach ,
Walter G. Thomas5, Ross A. D. Bathgate1,2*
1 Department of Biochemistry and Molecular Biology, The University of Melbourne, Victoria, Australia, 2 Florey Neuroscience Institutes, The University of Melbourne,
Victoria, Australia, 3 Department of Medicine (Austin Health), The University of Melbourne, Victoria, Australia, 4 Department of Anatomy and Neuroscience, The University
of Melbourne, Victoria, Australia, 5 School of Biomedical Sciences, University of Queensland, Queensland, Australia
Abstract
Relaxin-3, the most recently identified member of the relaxin peptide family, is produced by GABAergic projection neurons
in the nucleus incertus (NI), in the pontine periventricular gray. Previous studies suggest relaxin-3 is a modulator of stress
responses, metabolism, arousal and behavioural activation. Knockout mice and peptide infusions in vivo have significantly
contributed to understanding the function of this conserved neuropeptide. Yet, a definitive role remains elusive due to
discrepancies between models and a propensity to investigate pharmacological effects over endogenous function. To
investigate the endogenous function of relaxin-3, we generated a recombinant adeno-associated viral (rAAV) vector
expressing microRNA against relaxin-3 and validated its use to knock down relaxin-3 in adult rats. Bilateral stereotaxic
infusion of rAAV1/2 EmGFP miR499 into t
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