striatal medium-sized spiny neurons identification by nuclear staining and study of neuronal subpopulations in bac transgenic mice纹状体中型棘神经元核染色鉴定和研究的bac转基因小鼠中神经元的细胞亚群.pdfVIP

Striatal Medium-Sized Spiny Neurons: Identification by Nuclear Staining and Study of Neuronal Subpopulations in BAC Transgenic Mice 1,2,3. 1,2,3. 4 4 Miriam Matamales , Jesus Bertran-Gonzalez , Lucas Salomon , Bertrand Degos , Jean-Michel 4 1,2,3 ´ 1,2,3 1,2,3 Deniau , Emmanuel Valjent , Denis Herve , Jean-Antoine Girault * ´ ` ` 1 Inserm UMR-S 839, Paris, France, 2 Universite Pierre et Marie Curie (UPMC Paris-6), Paris, France, 3 Institut du Fer a Moulin, Paris, France, 4 Inserm U667, College de France, Paris, France Abstract Precise identification of neuronal populations is a major challenge in neuroscience. In the striatum, more than 95% of neurons are GABAergic medium-sized spiny neurons (MSNs), which form two intermingled populations distinguished by their projections and protein content. Those expressing dopamine D1-receptors (D1Rs) project preferentially to the substantia nigra pars reticulata (SNr), whereas those expressing dopamine D - receptors (D2Rs) project preferentially to the 2 lateral part of the globus pallidus (LGP). The degree of segregation of these populations has been a continuous subject of debate, and the recent introduction of bacterial artificial chromosome (BAC) transgenic mice expressing fluorescent proteins driven by sp