及胚胎组织块共移植对牛胚胎移植妊娠率影响.docxVIP

80.00%, higher than that of the control group(60.42%)，but there was no significantdifference（p0.05）.The pregnant rate sexed frozen embryo co-transfer with embryotrophoblast group was higher than that of the control group (51.15% vs 43.82%), which hadno significant difference (p0.05). The pregnant rate of sexed frozen embryo co-transferwith embryo mass group was 62.50%, which was higher than that of the control group(43.82%)(p0.05). The pregnant rate sexed fresh embryo co-transfer with fresh trophoblastgroup was higher than that of the control group (55.56% vs 46.15%), which had nosignificant difference (p0.05). Sexed fresh embryo co-transferred embryo co-transfer withembryo mass group had the best pregnant rate of 68.75%, which was higher than that of thecontrol group (46.15%), but there was no significant difference (p0.05).In summary, Bovine embryos were co-transferred with the grade of 3 embryo, embryotrophoblast or embryo mass can all improve the pregnancy rate of embryo transfer.Key words: Cattle; Embryo Tissues; Embryo Transfer; Pregnancy rate缩略词英文缩写IUFSHLHHCGGnRHCIDRMEBBEXBETEGPBSMPRbTPⅠINF-aTVsOXOTRJAK1TYK2FBSPVAPI英文全称International unitFollicle stimulating hormoneLuteinizing hormoneHuman chorionic gonadotropinGonadotropin releasing hormoneControlled drug release dispenserMorulaeEarly blastocystsBlastocystsExpand blastocystsEmbryo transferEthylene glycolDulbecco’s phosphate buffered salineMaternal pregnancy recognitionBovine trophoblastinⅠInterferon-alphaTrophoblastic vesiclesOxytocinOxytocin receptorJanus kinase 1Tyrosine kinase 2Fetal bovine serumPolyvinyl alcoholPropidium iodide中文全称国际单位促卵泡激素促黄体激素人绒毛膜促性腺激素促性腺激素释放激素孕酮阴道栓桑椹胚早期囊胚囊胚扩张囊胚胚胎移植乙二醇杜氏磷酸缓冲液妊娠识别牛滋养素Ⅰ干扰素-τ滋养泡催产素催产素受体杰氏激酶 1酪氨酸激酶 2胎牛血清聚乙烯醇碘化丙啶独创性声明本人声明所呈交的学位论文是本人在导师指导下进行的研究工作及取得的研究成果。据我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他人已经发表或撰写过的研究成果，也不包含为获得河北农业大学或其他教育机构的学位或证书而使用过的材料。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示谢意。学位论文作者签名：签字日期：年月日学位论文版权使用授权书本学位论文作者完全了解河北农业大学有关保留、使用学位论文的规定，有权保留并向国家有关部门或机构送交论文的复印件和磁盘，允许论文被查阅和借阅。本人授权河北农业