改良黄嘌呤氧化酶法测定动植物组织中SOD比活力.pdf

※分析检测 食品科学 2011, Vol. 32, No. 06 23 7 改良的黄嘌呤氧化酶法测定动植物组织中 SOD比活力 徐 东，赵 建，黄汉昌，文 镜* (北京联合大学生物活性物质与功能食品北京市重点实验室，北京 100 19 1) 摘 要：针对黄嘌呤氧化酶法测定动植物组织中SOD 活力存在干扰的问题，对方法加以改良。以使SOD 变性而不 影响干扰物质的干扰值为原则，通过测定酶活力及圆二色光谱寻找制备样品空白的最佳实验条件，设计经过加热的 样品空白；利用扣除法排除样品中其他物质对黄嘌呤氧化酶体系的干扰；通过加标回收实验以及对动植物组织的实 测，对改良前后的测定结果进行比较。结果表明：用改良的黄嘌呤氧化酶法可使动植物组织中SOD 活力的检测结 果更准确。 关键词：黄嘌呤氧化酶法；样品空白；检测超氧化物歧化酶活力 Determination of SOD Specific Activity in Animal and Plant Tissues by Improved Xanthine Oxidase Method XU Don g ，ZHAO Jian ，HUANG Han-chan g ，WEN Jin g* (Beij ing Key Laboratory of Bioactive Substances and Functional Foods, Beij ing Union University, Beij ing 100 19 1, China) Abstract：In order to minimize the interference in xanthine oxidase method for the determination of superoxide dismutase (SOD) activity in animal and plant tissues, the xanthine oxidase method was modified. The optimal experimental conditions for preparing bank samples were explored by measuring the activity and circular dichroism spectrum of SOD. A heated sample was designed as the experimental blank. Subtraction rule was used to eliminate the interference of other substances in xanthine oxidase system. Standard addition recovery experiments and actual determination of SOD activity in animal and plant tissues were used to compare the method with and without modification. Results indicated that the improved xanthine oxidase method could result in more accurate determination of SOD activity in animal and plant tissues. Key words ：xanthine oxidase method ；sample blank ；SOD activity determination 中图分类号：TS207.3 文献标识码：A 文章编号：1002-6630(20 11)06-0237-05