基于傅里叶变换红外光谱法的土茯苓真伪鉴别及溯源研究.PDFVIP

※分析检测 食品科学 2014, Vol.35, No.12 165 基于傅里叶变换红外光谱法的土茯苓真伪鉴别 及溯源研究 * 郑 捷，肖凤霞 ，林 励，郑宇翔，林靖然，雷胄熙 （广州中医药大学中药学院，广东广州 510006 ） 摘  要：利用傅里叶变换红外光谱法，建立红外指纹图谱和二阶导数图谱对30 批土茯苓样品及其常见混用品进行 鉴别分析，以期创立一种快速、有效鉴别土茯苓真伪的方法。研究确定了土茯苓的红外指纹特征及其与混用品的区 别，根据红外特征可很好区分土茯苓真品与其常见混用品；同时利用红外二阶导数图谱的差异，尝试对若干产自广 西与广东两地土茯苓进行了溯源分类探索。结果证明，利用红外指纹图谱结合二阶导数图谱的方法可实现对土茯苓 与其伪品间的区分鉴别，可为广西、广东两省区的土茯苓溯源研究提供参考，该方法专属性强、快速、稳定、对药 材无损伤。 关键词：土茯苓；红外指纹图谱；二阶导数图谱；真伪鉴别；溯源 Identification and Traceability of Smilax glabra Roxb. Rhizome by Fourier Transform Infrared Spectroscopy * ZHENG Jie, XIAO Feng-xia , LIN Li, ZHENG Yu-xiang, LIN Jing-ran, LEI Zhou-xi (College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China) Abstract: The aim of this study was to establish a simple and rapid method to identify the rhizome of Smilax glabra Roxb. and its adulterants from different sources. Fourier transform infrared spectroscopy (FTIR) combined with second derivative spectra was used to analyze 30 samples of Smilax glabra Roxb. rhizome and adulterants. The differences in their fingerprint characteristics were observed, which permit discrimination between Smilax glabra Roxb. rhizome and its adulterants from different sources. Also an attempt was made to classify Smilax glabra Roxb. samples from different geographic sources by second derivative spectra. This study demonstrated that infrared fingerprint combined with second derivative spectra can be applied in the identification or quality and traceability control of Smilax glabra Roxb. rhizome. This method is specific, rapid, stable and nondestructive. Key words: Smilax glabra Roxb.; infrared fingerprint;