βcatenin在白血病细胞系中表达探究.docVIP

βcatenin在白血病细胞系中表达探究 　 作者：麦玉洁 ，邱录贵 ，李增军 ，李新， 王国蓉， 毕业论文 于珍，徐燕 ，王亚非 ，李茜 【摘要】 本 研究 检测βcatenin在白血病/淋巴瘤细胞系中的表达，探讨其与白血病的关系。采用半定量RTPCR、免疫细胞化学和Western blot的 方法 检测βcatenin在白血病细胞系中的表达。结果表明: 白血病细胞系中βcatenin在转录水平上有广泛表达，其中：在U937、KG1a、 Jurkat、K562、Namalwa细胞中存在极高表达；在HEL、HUT78、Raji、Daudi、CEM中有中等表达；而在LCLH、HL60、NB4、J61、Ramos细胞中βcatenin mRNA呈相对较低水平的表达。蛋白表达水平与mRNA表达水平结果一致。所检测的细胞系中，胞核中均可见βcatenin的表达，但程度不一;在U937、K562、KG1a 和Jurkat细胞的胞核内可见大量分布。结论: βcatenin作为WNT/βcatenin信号转导途径中重要的“调节子”，其在白血病细胞中的过量表达提示WNT/βcatenin信号转导途径可能在白血病细胞中有异常激活。 毕业论文 【关键词】 白血病细胞系 Expression of βCatenin in Leukemic Cell Lines Abstract This study was aimed to investigate the expression of βcatenin in leukemic cell lines and its relationship with pathogenesis of leukemia, semiquantitative RTPCR and Western blot were performed to detect the expression of βcatenin in a panel of 15 human hematopoietic cell lines (U937, KG1a, Jurkat, K562, Namalwa, HEL, HUT78, Raji, Daudi, CEM, LCLH, HL60, NB4, J61, Ramos). Immunocytochemistry was performed in some of these cell lines to detect the location of βcatenin. The results showed that the βcatenin gene was widely expressed in most leukemic cell lines in various degree, the high expression of βcatenin was found is U937, KG1a, Jurkat, K562 and Namalwa cells, middle erpression of βcatenin was observed in HEL, HUT78, Raji, Daudi and CEM cells, lower expression of βcatenin was observed in LCLH, HL60, NB4, J61 and Ramos cells. The expression level of βcatenin protEin was identical to the expression level of βcatenin mRNA. The expression of βcatenin could be found in nuclEI of all cells mentioned above, but their levels were different belween them. Abundant βcatenin also could be observed in nuclei of some leukemic cells by immunocytochemistry. It is concluded that overexpression of βcatenin in leukemia cells, as a key mediator of Wnt signaling transduction pathway, indicates that the Wnt signaling transduction pathway may be aberrantly