中风芪红利水胶囊对脑缺血大鼠脑组织影响.docVIP

中风芪红利水胶囊对脑缺血大鼠脑组织影响 　 作者：路永坤，冯国磊，关运祥，秦秀德，王新志 【摘要】 目的评价中风芪红利水胶囊对急性脑缺血大鼠脑组织中兴奋性氨基酸及水含量的影响。方法采用左侧大脑中动脉阻塞模型，72只清洁级雄性Wistar大鼠随机分为假手术组，模型组，对照组（步长脑心通胶囊组），中风芪红利水胶囊高、中、低剂量组。造模成功12 h后处死。采用高效液相色谱法测量脑组织中的谷氨酸(Glu)、天门冬氨酸(Asp)含量。通过脑组织干重与湿重之比，算出含水量。结果芪红利水胶囊各剂量组脑组织中Glu含量较缺血组显著降低（Plt;0.05）；芪红利水胶囊高、中、低剂量治疗组脑组织中天门冬氨酸含量较缺血模型组下降显著(P=0.000,P=0.031,P=0.05)，芪红利水胶囊各剂量组脑组织含水量较缺血模型组下降显著(Plt;0.01)。结论中风芪红利水胶囊具有抗大鼠急性脑缺血损伤的作用。其作用机制与降低脑组织中谷氨基酸、天门冬氨酸及脑组织含水量有关。 【关键词】 急性脑缺血；大鼠；兴奋性氨基酸；脑水肿；中风芪红利水胶囊 　　Abstract：ObjectiveTo assess the effects of the Zhongfeng Qihong Lishui capsule (QHLSC) on excitatory amino acid (EAA) and water content in brain tissue of rats following acute brain ischemia.MethodsThe left middle cerebral artery occlusion (LLMCAO) model was employed. Seventy two male Wistar rats were randomly divided into the sham-operation group, model group, control group (Buchang Naoxin Tong group) and high dose, middle dose, low dose groups of QHLSC. Drugs and saline were administered respectively, twice a day, for 3 consecutive days before LMCAO and once 2 hours after LMCAO. The rats were sacrificed twelve hours after LMCAO. Glu (glutamate), Asp (aspartate) were measured through the method of HPLC (high performance liquid chromatography). Water content was assessed by comparison of the wet weight against the dry weight. ResultsThe contents of Glu, Asp and water in the brain tissues of ischemia group increased greatly compared with that of the sham operation group (Plt;0.01). (2) The content of Glu in the brain tissues of rats in the groups treated with QHLSC decreased significantly compared with that of the ischemia group (Plt;0.05); the level of Asp in the brain tissues of rats in the high dose group, middle dose group, low dose group treated with QHLSC decreased significantly compared with that of the ischemia group (P=0.000,P=0.031,P=0.05 respectively). The content of water in the brain tissue of rats in the groups treated with QHLSC decreased significantly compared with that of the ischemia group