二烯丙基二硫化物对HL-60白血病细胞株VEGF表达及分泌影响.docVIP

二烯丙基二硫化物对HL-60白血病细胞株VEGF表达及分泌影响 　 作者：谢熠，范自力，姚晨姣，谭三勤，赵亚丽 【摘要】 为了研究二烯丙基二硫化物（diallyl disulfide，DADS）对白血病HL-60细胞株VEGF mRNA的表达及VEGF蛋白分泌的作用，并从影响VEGF生成的角度探讨DADS的抗白血病机制，应用ELISA法检测药物作用前后白血病HL-60细胞培养上清液中VEGF蛋白的含量； RT-PCR法检测药物作用前后白血病HL-60细胞VEGF mRNA的相对含量。结果表明： HL-60细胞中均有VEGF mRNA的表达和VEGF蛋白的分泌；与空白对照相比，DADS 3个浓度组（0.625，1.25，2.5 μg/ml）作用HL-60细胞48小时和72小时均能下调HL-60细胞VEGF mRNA的表达及VEGF蛋白的分泌（Plt;0.01）。3个浓度组间差异显著（Plt;0.01），其下调HL-60细胞VEGF mRNA的表达及VEGF蛋白的分泌效果与浓度相关（rgt;0.9，Plt;0.01）。结论： DADS可能通过抑制VEGF mRNA的表达及VEGF蛋白的分泌发挥抗白血病效应。 【关键词】 二烯丙基二硫化物 　　Effect of Diallyl Disulfide on Expression and Secretion of VEGF in HL-60 Leukemic Cells 　　Abstract The study was aimed to investigate the expression of VEGF mRNA and VEGF protein in HL-60 cells treated with diallyl disulfide (DADS)，and to explore the antileukemic mechanism of DADS in respect of VEGF production.Semi-quantitative RT-PCR and ELISA were used to detect the expression of VEGF mRNA and secretion of VEGF protein in HL-60 cell lines treated by DADS respectively. The results showed that the expression of VEGF mRNA and secretion of VEGF protein were found in HL-60 cells.The expression of VEGF mRNA and secretion of VEGF protein in HL-60 cells could be down regulated by treatment with 0.625，1.25，and 2.5 μg/mL DADS for 48 and 72 hours and the effects had a dose dependent relationship（rgt;0.9，Plt;0.01）.The differences between DADS treated HL-60 cell groups and the control group were statistically significant （Plt;0.01），there were also statistically significant differences among three DADS-treated HL-60 cell groups（Plt;0.05）. It is concluded that DADS effectively inhibits the proliferation of human leukemia cell line HL-60 cells; DADS exerts its antileukemic effects by reduction of the expression of VEGF mRNA and VEGF protein secretion. 　　Key words diallyl disulfide; HL-60 cell; leukemia; vascular endothelial growth factor 全反式维甲酸（ATRA）已成为治疗急性早幼粒白血病的首选药物，但也有其局限性，并且还会发生维甲酸综合征不良反应，因此寻找毒副作用低的抗白血病的药物仍是当前研究的重要课题。已有研究证明，大蒜中的一种脂溶性的有效成分二烯丙基二硫化物（diallyl di