喉鳞癌组织中SPARC基因差异表达.docVIP

喉鳞癌组织中SPARC基因差异表达 　 作者：车轶群, 王海, 史雪冬，齐军, 郭健, 刘芝华 【摘要】 目的探讨富含半胱氨酸的酸性蛋白(SPARC)在喉鳞癌的增殖、侵润和转移中的作用及指导意义。方法用半定量逆转录聚合酶链反应（RTPCR）分析SPARC在不同的喉鳞癌组织和其相邻的正常粘膜组织的差异表达量。利用免疫组化技术研究SPARC在喉鳞癌组织和其相邻的正常粘膜组织的表达差异,同时对蛋白表达进行定位。结果RTPCR 分析SPARC在20对配对标本中的表达, 在其中16对喉鳞癌组织中的表达明显高于其相应的正常粘膜组织, 在4对中差异表达不明显, R=80.0%(有差异的例数/总例数)。免疫组化技术分析了30对配对喉鳞癌手术标本的石蜡切片(肿瘤组织和正常粘膜组织), 其中25对SPARC蛋白在癌巢细胞中的表达高于相邻的正常粘膜组织, 5例在癌巢细胞中和相邻的正常粘膜组织内表达不明显，R=83.3%。同时对SPARC蛋白在不同分化程度的喉癌组织中的表达进行分析, 发现SPARC在中低分化喉癌中的表达与高分化喉癌无显著差异(Pgt;0.05)。而在有淋巴结转移的喉癌的表达比无淋巴结转移的高（P=0.036）。细胞核和细胞浆均有表达。结论通过mRNA和蛋白水平分析得出，SPARC和喉鳞癌的发生密切相关, 喉鳞癌淋巴结转移时SPARC表达升高。 【关键词】 SPARC； 喉鳞癌； 基因表达； 免疫组化 　　Department of Clinical Laboratory, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China; Department of Clinical Laboratory, ChinaJapan Union Hospital of Jilin University;National Center for Clinical Laboratory, Ministry of Health of the PR. China;National Laboratory of Molecular Oncology, Cancer Institute amp; Hospital, Chinese Academy of Medical Sciences amp; Peking Union Medical College 　　Abstract：Objective To investigate the role of secreted protein, acidic and rich in cysteine (SPARC) in proliferation and metastasis of laryngeal squamous cell carcinoma (LSCC). MethodsThe differential gene expression of SPARC between laryngeal squamous cell carcinoma and its corresponding normal laryngeal mucosa from 20 patients was analyzed by semiquantitative reverse transcriptionpolymerase chain reaction (RTPCR). The differential protein expression of SPARC between laryngeal squamous cell carcinoma and its corresponding normal laryngeal mucosa was analyzed by immunohistochemistry (IHC) analysis using well characterized monoclonal antibodies. ResultsThe gene expression of SPARC in LSCC by RTPCR was higher than that in the corresponding normal laryngeal mucosa (16/20). The expression of SPARC protein was significantly upregulated in LSCC compared with the corresponding normal laryngeal mucosa in 30 couple cases, not