复方蜥蜴散对慢性萎缩性胃炎模型大鼠肿瘤坏死因子-α及胃黏膜病.docVIP

复方蜥蜴散对慢性萎缩性胃炎模型大鼠肿瘤坏死因子-α及胃黏膜病 　 作者：张静霜，朱西杰，朱微微，陈健茂，吴璟，李卫强 【摘要】 　　目的观察复方蜥蜴散对慢性萎缩性胃炎（Chronic Atrophic Gastritis，CAG）模型大鼠肿瘤坏死因子-α（TNF－α）及胃黏膜病理形态学的影响,探讨其治疗CAG的作用机制。方法将90只SD雄性大鼠随机分为正常组，模型组，复方蜥蜴散大、中、小剂量组及维酶素组,除正常组以外，均采取55℃热盐水、2%水杨酸、20 mmol/L脱氧胆酸钠3个致萎缩因素配合饥饱失常造成CAG模型大鼠,分别运用复方蜥蜴散大、中、小剂量及维酶素治疗,观察各组大鼠血清TNF－α的含量及胃黏膜的病理学形态。结果蜥蜴散大、中、小剂量组血清中TNF-α含量显著低于模型组（Plt;0.01），蜥蜴散大、中剂量组血清中TNF-α含量显著低于维酶素组（Plt;0.05）。各组胃黏膜组织形态学观察，复方蜥蜴散组较模型组及维酶素组有明显改善。结论复方蜥蜴散可以使CAG模型大鼠血清中TNF－α含量降低，能够明显改善CAG模型大鼠胃黏膜的炎症、萎缩性病变,对CAG模型大鼠有明显的治疗作用。 【关键词】 慢性萎缩性胃炎　复方蜥蜴散　肿瘤坏死因子-α　病理组织学 　　Abstract：ObjectiveTo investigate the effect of compound lizards powder(TCLP) ,a compound Chinese medicine ,on the tumor necrosis factor－α(TNF－α), and gastric mucosa pathological change in rat model of Chronic Atrophic Gastritis（CAG）. and the mechanisms.MethodsNinety male SD rats were randomly divided into six groups:the normal control group,the model group，the Vitacoenzyme control group，the low-dose,middle-dose and high-dose TCLP groups. Except normal control group, other groups took 55℃ hot water which contained 150g/L NaCl,2% Salieylas and 20mmol/L sodium deoxyeholate,combined with overeating and hungering.All rats were treated with different medicines，after a month the rats were sacrificed，and their gastric mucosa and serum were collected to detect the serum level of TNF－α and observe the histology mucosa changes by microscope.ResultsCompared with model group，the serum level of TNF－α in the rats of all TCLP groups was significantly lowered(Plt;0.01). Compared with Vitacoenzyme control group the serum level of TNF－α in the rats of middle-dose and high-dose TCLP groups was significantly lowered(Plt;0.05), and the pathological changes were significant. ConclusionTCLP can significantly adjust level of TNF－α,and has protective function against the gastric mucosal injury. 　　Key words： Chronic Atrophic Gastritis (CAG); The compound lizards powder; Tumor Putrescence Factor (TNF); Pathological histology 　　慢性萎缩性胃炎(Chronic Atrophic Gastritis，C