糖心平胶囊对糖尿病大鼠心肌超微结构、血管紧张素Ⅱ及其1型受体.docVIP

糖心平胶囊对糖尿病大鼠心肌超微结构、血管紧张素Ⅱ及其1型受体 　 作者：李敏, 倪青, 楚小燕, 王兆礼, 林兰 【摘要】 目的：探讨糖心平胶囊对糖尿病大鼠心肌病变的保护作用及机制。方法：80只大鼠通过腹腔注射链脲佐菌素建立糖尿病模型，并随机分为模型组、格华止组、开搏通组和糖心平胶囊大、中、小剂量治疗组，灌胃给药8周，并以10只正常大鼠作为正常对照组。分别采用放射免疫法和逆转录聚合酶链式反应法检测给药后各组大鼠心肌组织中Ang Ⅱ含量和AT1R mRNA表达水平，透射电子显微镜检测心肌超微结构的改变。结果：模型组、格华止组和糖心平中、小剂量组心肌组织中Ang Ⅱ含量和AT1R mRNA表达较正常对照组显著增高（P＜0.05，P＜0.01）。糖心平大剂量组和开搏通组心肌组织中Ang Ⅱ含量和AT1R mRNA表达低于模型组（P＜0.05，P＜0.01）。模型组心肌超微结构与正常对照组相比，心肌线粒体体积轻度增大。所有治疗组心肌超微结构较模型组有明显改善。结论：糖心平胶囊可能通过调整心肌局部肾素血管紧张素系统的紊乱对糖尿病心肌病变产生保护作用。 【关键词】 糖心平胶囊; 糖尿病并发症; 血管紧张素Ⅱ; 血管紧张素Ⅱ1型受体; 心肌疾病; 大鼠 Methods: The model of diabetes mellitus was induced by streptozotocin (STZ) injection in rats. And the rats with diabetes mellitus were randomly divided into untreated group, metformin group, captopril group, and low, medium and highdose Tangxinping group; the other ten normal rats were used as normal controls. After eightweek treatment, all rats were sacrificed to detect angiotensin Ⅱ (Ang Ⅱ) protein content and angiotensin Ⅱ type 1 receptor (AT1R) mRNA expression in myocardial tissues by radioimmunoassay and reverse transcription polymerase chain reaction (RTPCR) respectively. The ultrastructural change in myocardial tissues was detected by transmission electron microscopy. Results: The results of radioimmunoassay and RTPCR showed that the content of Ang Ⅱ protein and the expression of AT1R mRNA in myocardial tissue of the untreated group was significantly increased and more than those in normal control (P＜0.05, P＜0.01). Only the content of Ang Ⅱ and expression of AT1R mRNA in highdose Tangxinping group and captopril group were significantly lower than those in the untreated group (P＜0.05, P＜0.01). The transmission electron microscopy showed that volume of mitochondrion in myocardial tissue in the untreated group was bigger than that in normal control group. Also the volume of mitochondrion was improved in 5 treated groups, but had no significant difference when compared with the untreated group. Conclusion: Tangxinping may adjust the