苦石莲皮普通粉及微粉挥发性成分对比探究.docVIP

苦石莲皮普通粉及微粉挥发性成分对比探究 　 作者：袁月玲 霍昕 高玉琼 刘莹 丁丽娜 杨迺嘉 【摘要】 　　目的研究苦石莲皮中的挥发性成分，并对比普通粉和微粉中挥发性成分异同。方法利用有机溶剂水蒸气蒸馏法提取苦石莲皮中的挥发成分，用气相色谱质谱联用（GC-MS）进行测定，色谱柱为HP-5MS 5% Phenyl Methyl Siloxane弹性石英毛细管柱(30 m×0.25 mm×0.25 μm)，结合计算机检索技术对分离的化合物进行结构鉴定，应用色谱峰面积归一化法计算各成分的相对百分含量。结果苦石莲皮普通粉中分离出115个化学成分，鉴定出63个化学成分，检出率为54.7％，其中相对百分含量大于2.0％有5种；微粉中分离出119个化学成分，鉴定出64个化学成分，检出率为53.7％，其中相对百分含量大于2.0％有9种。两种粉末挥发性成分中有54种相同成分，微粉中含量高于普通粉中相应成分的有28种，其中含量最高的分别是柠檬烯（5.311%，普通粉）和亚油酸甲酯（3.388％微粉）。结论该实验首次对苦石莲皮普通粉和微粉挥发性成分进行对比研究。 【关键词】 苦石莲； 有机溶剂水蒸气蒸馏； 挥发性成分； 气相色谱-质谱联用 　　Abstract：ObjectiveTo study the chemical constituents of volatile constituents from the shell of Caesalpinia minax Hance. MethodsThe chemical compositions of the volatile constituents of the plant which were obtained by extraction with water added by residual solvent were analyzed by GC-MS equipped with a elastic quartz capillary column-HP-5MS 5% Phenyl Methyl Siloxane (30 m×0.25 mm×0.25 μm). The constituents were identified by their mass spectra. The relative percentage of the volatile constituents was calculated from the GC peak areas.ResultsOne hundred and fifteen kinds of chemical constituents from the ordinary powder of the shell of Caesalpinia minax Hance were separated and sixty-three of them, which accounts for 54.7%, were characterized. There were five kinds whose relative contents were more than 2.0%; One hundred and nineteen kinds of chemical constituents from the micronized powder of the shell of Caesalpinia minax Hance were separated and sixty-four of them, which accounted for 53.7% , were characterized. There were nine kinds whose relative contents were more than 2.0%; There were the same of fifty-four kinds chemical constituents in the two kinds of powder, of which the contents of twenty-eight constituents from the micronized powder were more than those in the ordinary powder. The most relative contents were Limonene(5.311% ordinary powder) and Methyl linoleate(3.388%, micronized powder ) . ConclusionThis paper reports, for the first time,