菱角皮提取物中鞣酸类化合物含量测定及其指纹图谱探究.docVIP

菱角皮提取物中鞣酸类化合物含量测定及其指纹图谱探究 　 作者：符少莲，周光雄，黄美燕 【摘要】 目的建立菱角皮醋酸乙酯提取物中鞣质类化合物的HPLC含量测定方法，并对提取物HPLC指纹图谱条件进行研究分析。方法采用反相C18柱（4.6 mm×200 mm，5 μm），甲醇－0.5%乙酸水溶液为流动相，梯度洗脱，柱温为25℃，流速为0.8 ml/min，在276 nm下进行检测。结果菱角皮醋酸乙酯提取物中鞣质类化合物1,2,6-三没食子酰-β-D-葡萄糖的含量为85.7 mg/g，加样回收率为92.49%；1,2,4,6-四没食子酰-β-D-葡萄糖的含量为244 mg/g，加样回收率为96.41%；初步探讨了菱角皮提取物的指纹图谱条件。结论采用高效液相法测定菱角皮醋酸乙酯提取物中鞣质类化合物的含量，简便、快速、准确；初步得到分离菱角皮提取物各特征峰的色谱条件，为以后建立指纹图谱和制定菱角皮药材及其活性提取物的质量标准提供了依据。 【关键词】 菱； 果皮； 鞣酸； 高效液相色谱； 指纹图谱 　　Abstract：Objective To establish determination method of tannin compounds from bioactive ethyl acetate extract of the fruit crust of Trapa bispinosa Roxb by HPLC, and to study on analysis conditions of HPLC fingerprint of the extract. MethodsUse reverse phase C18 column（4.6 mm×200 mm，5 μm）, detection wave-length at 276 nm, column temperature at 25℃, flow rate at a 0.8 ml/min, and gradient aqueous methanol solution containing 0.5% acetic acid as a mobile phase.ResultsThe determination of tannic acid compound 1, 2, 6-tri-galloyl-β- D-glucose from the extract was 85.7mg/g, and the average recoveries was 92.49%; the determination of tannic acid compound 1, 2, 3, 6-tetra-galloyl-β-D -glucose was 244 mg/g, and the average recoveries was 96.41%; a preliminary study on conditions of HPLC fingerprint for the bioactive extract was carried out. ConclusionThe determination method for the tannin compounds from ethyl acetate extract of the fruit crust of the plant by HPLC is simple, fast and accurate. Chromatographic conditions of ethyl acetate extract of the plant was initially obtained, which provide the basis for establishment of the HPLC fingerprint and making quality standard of the crude drug and its bioactive extract from the plant. 　　Key words：Trapa bispinosa Roxb; Fruit crust; Tannin; HPLC；Fingerprint 　　菱Trapa bispinosa Roxb，一年生浮水或半挺草本，属菱科。我国有15种和11变种，产于全国各地，以长江流域亚热带地区分布与栽培最多［1］。其性甘味凉，无毒，入肠、胃二经，有清热祛暑、除湿祛风、益气健脾之功［2］。菱壳，为菱科植物菱或其同属植物的果皮。其作为药物始载于《本草纲目拾遗》。据《中华药海》所载，其性味微苦、涩，凉，入肺、脾、大肠三经，具有解毒疗疮、涩汤止泻、清化湿热的功效［2］。 　　经查阅文献得知，东北菱T. man