葎草多糖超声提取及抑菌活性探究.docVIP

葎草多糖超声提取及抑菌活性探究 　【摘要】 　　目的优化葎草多糖的超声辅助提取工艺，并研究其抑菌活性。方法以多糖中的葡萄糖含量为指标，对超声波功率、料液比和超声时间进行L9（34）正交实验，确定超声辅助提取葎草粗多糖的最佳工艺。用DE-52交换柱对葎草粗多糖进行分离，以滤纸片法研究葎草粗多糖和多糖分离组分的抑菌能力。结果以水为提取溶剂，料液比1∶28（g/ml），超声功率120W，超声90 min提取2次，葎草多糖的得率为0.755％。葎草粗多糖的抑菌效果具有一定的剂量依赖性；各多糖组分中HSP-Ⅰ、HSP-Ⅲ、HSP-Ⅳ发挥主要抑菌作用，其中HSP-Ⅰ效果最显著，HSP-Ⅱ抑菌效果不明显，且各组分间表现出协同关系。结论超声波辅助提取葎草多糖方法可行，得率高。葎草多糖具有抑菌活性。 【关键词】 葎草　多糖　分离　抑菌活性 　　Abstract：ObjectiveTo optimize the ultrasound-assisted extraction method and to study the antibacterial activity of polysaccharides from Humulus scandens. MethodsTaking the content of glucose as observation index, we optimized the extraction process by L9（34）orthogonal experiment. The Humulus scandens polysaccharides (HSP) was purified into HSP-Ⅰ, HSP-Ⅱ, HSP-Ⅲ and HSP-Ⅳ by ion exchange chromatography with DE-52. The antibacterial activity of each component was tested by means of filter paper.ResultsThe optimal extraction conditions were obtained as follows: water refluxing, a ratio of solid -liquid as 1g:28ml,120W for the intension of ultrasonic power, extracting time 90min and extracting two times. Under aforesaid optimization conditions, the yield of polysaccharides was 0.755%. Antibacterial experiments indicated that the inhibitory effect of crude polysaccharides showed dose-dependent. Among the isolated components, HSP-Ⅰ, HSP-Ⅲ, HSP-Ⅳ played main antibacterial roles and HSP-Ⅱ was ineffective. It was cooperative with different isolated components. ConclusionThe yield of Humulus Scandens polysaccharides is high with ultrasound-assisted extraction method. The Humulus scandens polysaccharides has obvious antibacterial action. 　　Key words：Humulus scandens; Polysaccharides; Isolation; Antibacterial activity 葎草Humulus scandens (Lour.)Merr.又名拉拉藤，拉拉秧，一年生或多年生草本植物，全国各地几乎均有分布。葎草生长旺盛，富含营养，既可以作为饲料，提高饲喂动物的生长状况，又可以药用，有清热解毒、利尿消肿之功效［1］。据文献报道，葎草中含有木犀草素、葡萄糖苷、胆碱、挥发油、鞣质、树脂、天门冬酰胺等物质［2］，其提取物具有抗结核菌的功效［3］，因而渐渐引起人们的研究兴趣。近年对于葎草中的木犀草素、黄酮类物质的提取已有少量研究［4，5］，但对葎草中多糖类物质的提取和活性研究还未见报道。本文采用超声波辅助提取方法制备葎草总多糖，并对多糖的抑菌活性进行研究，以期为这一优势植物资源的开发利用