absolute estimation of initial concentrations of amplicon in a real-time rt-pcr process绝对的估计初始浓度实时rt - pcr扩增子的过程.pdfVIP

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absolute estimation of initial concentrations of amplicon in a real-time rt-pcr process绝对的估计初始浓度实时rt - pcr扩增子的过程.pdf

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absolute estimation of initial concentrations of amplicon in a real-time rt-pcr process绝对的估计初始浓度实时rt - pcr扩增子的过程

BMC Bioinformatics BioMed Central Methodology article Open Access Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process 1 2,3 2,4 2 Marjo V Smith* , Chris R Miller , Michael Kohn , Nigel J Walker and Chris J Portier2 Address: 1Constella Group, Suite 200, 2605 Meridian Pky, Durham, NC, USA, 2National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA, 3Now at Applied Biosystems Inc, USA and 4Now deceased Email: Marjo V Smith* - msmith@; Chris R Miller - MillerCR@; Michael Kohn - kohn@; Nigel J Walker - Walker3@; Chris J Portier - Portier@ * Corresponding author Published: 23 October 2007 Received: 31 January 2007 Accepted: 23 October 2007 BMC Bioinformatics 2007, 8:409 doi:10.1186/1471-2105-8-409 This article is available from: /1471-2105/8/409 © 2007 Smith et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Since real time PCR was first developed, several approaches to estimating the initial quantity of template in an RT-PCR reaction have been tried. While initially only the early thermal cycles corresponding to exponential duplication were used, late