phytochemical and cytotoxic investigations of alpinia mutica rhizomes植物化学的和细胞毒性成分的调查mutica根状茎.pdfVIP

Molecules 2011, 16, 583-589; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Phytochemical and Cytotoxic Investigations of Alpinia mutica Rhizomes Sri Nurestri Abdul Malek 1,*, Chung Weng Phang 1, Halijah Ibrahim 1, 2 1 Norhanom Abdul Wahab and Kae Shin Sim 1 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia 2 Centre for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia * Author to whom correspondence should be addressed; E-Mail: srimalek@.my; Tel.: +603 Fax: +603 Received: 2 December 2010; in revised form: 31 December 2010 / Accepted: 7 January 2011 / Published: 14 January 2011 Abstract: The methanol and fractionated extracts (hexane, ethyl acetate and water) of Alpinia mutica (Zingiberaceae) rhizomes were investigated for their cytotoxic effect against six human carcinoma cell lines, namely KB, MCF7, A549, Caski, HCT116, HT29 and non-human fibroblast cell line (MRC 5) using an in vitro cytotoxicity assay. The ethyl acetate extract possessed high inhibitory effect against KB, MCF7 and Caski cells (IC50 values of 9.4, 19.7 and 19.8 µg/mL, respectively). Flavokawin B (1), 5,6-dehydrokawain (2), pinostrobin chalcone (3) and alpinetin (4), isolated from the active ethyl acetate extract were also evaluated for their cytotoxic activity