原创力文档quantitation of pseudomonas aeruginosa in wound biopsy samples from bacterial culture to rapid `real-time polymerase chain reaction铜绿假单胞菌的定量伤口活检样本细菌培养快速实时聚合酶链反应.pdfVIP
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quantitation of pseudomonas aeruginosa in wound biopsy samples from bacterial culture to rapid `real-time polymerase chain reaction铜绿假单胞菌的定量伤口活检样本细菌培养快速实时聚合酶链反应
/content/4/4/255
Primary research
Quantitation of Pseudomonas aeruginosa in wound biopsy
samples: from bacterial culture to rapid ‘real-time’ polymerase
chain reaction
† †‡
Jean-Paul Pirnay* , Daniel De Vos , Luc Duinslaeger*, Pascal Reper*,
Christian Vandenvelde*, Pierre Cornelis† and Alain Vanderkelen*
*Queen Astrid Military Hospital, Neder-Over-Heembeek, † Flanders Interuniversity Institute of
Biotechnology, Sint-Genesius-Rode, and ‡Innogenetics, Neder-Over-Heembeek, Belgium
Received: 9 January 2000 Crit Care 2000, 4:255–261
Revisions requested: 29 February 2000
The electronic version of this article can be found online at
Revisions received: 8 June 2000
/content/4/4/255
Accepted: 14 June 2000
Published: 7 July 2000 © Current Science Ltd
Statement of findings
We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid
thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples.
This method produced a linear quantitative detection range of 7 logs, with a lower detection
limit of 103 colony-forming units (CFU)/g tissue or a few copies per reaction. The time from
sample collection to result was less than 1 h. RTD-PCR has potential for rapid quantitative
detection of pathogens in critical care patients, enabling early and individualized treatment.
Keywords: burn wound, polymerase chain reaction,
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