原创力文档regulation of callose synthase activity in situ in alamethicin-permeabilized arabidopsis and tobacco suspension cells监管的胼胝质合成酶活性原位alamethicin-permeabilized拟南芥和烟草悬浮细胞.pdfVIP
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regulation of callose synthase activity in situ in alamethicin-permeabilized arabidopsis and tobacco suspension cells监管的胼胝质合成酶活性原位alamethicin-permeabilized拟南芥和烟草悬浮细胞
BMC Plant Biology BioMed Central
Research article Open Access
Regulation of callose synthase activity in situ in
alamethicin-permeabilized Arabidopsis and tobacco suspension cells
Mari Aidemark, Carl-Johan Andersson, Allan G Rasmusson and
Susanne Widell*
Address: Department of Cell and Organism Biology, Lund University, Sölvegatan 35, SE-223 62 Lund, Sweden
Email: Mari Aidemark - mari.Aidemark@cob.lu.se; Carl-Johan Andersson - cajo.andersson@;
Allan G Rasmusson - Allan.Rasmusson@cob.lu.se; Susanne Widell * - Susanne.Widell@cob.lu.se
* Corresponding author
Published: 12 March 2009 Received: 3 October 2008
Accepted: 12 March 2009
BMC Plant Biology 2009, 9:27 doi:10.1186/1471-2229-9-27
This article is available from: /1471-2229/9/27
© 2009 Aidemark et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: The cell wall component callose is mainly synthesized at certain developmental
stages and after wounding or pathogen attack. Callose synthases are membrane-bound enzymes
that have been relatively well characterized in vitro using isolated membrane fractions or purified
enzyme. However, little is known about their functional properties in situ, under conditions when
the cell wall is intact. To allow in situ investigations of the
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